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• W3021515970 abstract "Abstract Background Paralytic shellfish toxins (PST) are a significant problem for the Tasmanian shellfish and Southern Rock Lobster (Jasus edwardsii) industries, and the introduction of a rapid screening test in the monitoring program could save time and money. Objective The aim was to perform a single-laboratory validation of the Neogen rapid test for PST in the hepatopancreas of Southern Rock Lobster. Methods The AOAC INTERNATIONAL guidelines for the validation of qualitative binary chemistry methods were followed. Three different PST profiles (mixtures) were used, of which two were commonly found in naturally contaminated lobster hepatopancreas (high in gonyautoxin 2&3 and saxitoxin), and the third toxin profile was observed in a few select animals (high in gonyautoxin 1&4). Results The Neogen test consistently returned negative results for non-target toxins (selectivity). The probability of detection (POD) of PST in the lobster hepatopancreas using the Neogen test increased with increasing PST concentrations. POD values of 1.0 were obtained at ≥0.57 mg STX-diHCl eq/kg in mixtures 1 and 2, and 0.95 and 1.0 for mixture 3 at 0.79 and 1.21 mg STX-diHCl eq/kg, respectively, with a fitted POD of 0.98 for 0.80 mg STX-diHCl eq/kg. The performance of the Neogen test when using four different production lots (ruggedness) showed no significant differences. Conclusions The results of the validation study were satisfactory and the Neogen test is being trialed within the Tasmanian PST monitoring program of Southern Rock Lobster. Highlights The Neogen rapid kit was successfully validated for the detection of PST in Southern Rock Lobster hepatopancreas." @default.
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• W3021515970 date "2020-05-01" @default.
• W3021515970 modified "2023-10-17" @default.
• W3021515970 title "Detection of Paralytic Shellfish Toxins in Southern Rock Lobster <i>Jasus edwardsii</i> Using the Qualitative Neogen™ Lateral Flow Immunoassay: Single-Laboratory Validation" @default.
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• W3021515970 doi "https://doi.org/10.1093/jaocint/qsz029" @default.
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