Matches in SemOpenAlex for { <https://semopenalex.org/work/W3021713899> ?p ?o ?g. }
Showing items 1 to 56 of
56
with 100 items per page.
- W3021713899 endingPage "58" @default.
- W3021713899 startingPage "53" @default.
- W3021713899 abstract "Whole cells of Lactobacillus delbrueckii subsp. bulgaricus ACA DC235 are able to hydrolyse casein. This proteolytic activity is greatly enhanced when cells are grown in milk rather than in a peptide-rich synthetic medium such as De Man-Sharp-Rogosa. A significant part of the caseinolytic activity can be extracted by treating the intact cells with lysozyme, which suggests that the enzyme(s) involved are associated with the cell wall. The soluble lysozyme extract has been partially fractionated by ultrafiltration using different membranes. Biphasic kinetics of irreversible thermal denaturation, partial inactivation by various agents, and selective reactivation by zinc ions indicated that the overall caseinolytic activity was due to two distinct enzymes. The first one was rapidly inactivated upon heating, inhibited by EDTA, reactivated by Zn2+ ions, and was probably a zinc-dependent metalloprotease. The other one was more stable towards thermal inactivation, inhibited by phenylmethanesulfonyl fluoride, insensitive to N-ethylmaleimide, activated by Ca2+ ions, and was probably a serine protease." @default.
- W3021713899 created "2020-05-13" @default.
- W3021713899 creator A5035578911 @default.
- W3021713899 creator A5036398846 @default.
- W3021713899 creator A5057266962 @default.
- W3021713899 date "1995-04-15" @default.
- W3021713899 modified "2023-09-23" @default.
- W3021713899 title "The presence of two proteinases associated with the cell wall of Lactobacillus bulgaricus" @default.
- W3021713899 doi "https://doi.org/10.1016/0378-1097(95)00082-g" @default.
- W3021713899 hasPublicationYear "1995" @default.
- W3021713899 type Work @default.
- W3021713899 sameAs 3021713899 @default.
- W3021713899 citedByCount "1" @default.
- W3021713899 crossrefType "journal-article" @default.
- W3021713899 hasAuthorship W3021713899A5035578911 @default.
- W3021713899 hasAuthorship W3021713899A5036398846 @default.
- W3021713899 hasAuthorship W3021713899A5057266962 @default.
- W3021713899 hasConcept C125235067 @default.
- W3021713899 hasConcept C185592680 @default.
- W3021713899 hasConcept C2780206646 @default.
- W3021713899 hasConcept C2780437316 @default.
- W3021713899 hasConcept C523546767 @default.
- W3021713899 hasConcept C54355233 @default.
- W3021713899 hasConcept C55493867 @default.
- W3021713899 hasConcept C86803240 @default.
- W3021713899 hasConcept C89423630 @default.
- W3021713899 hasConceptScore W3021713899C125235067 @default.
- W3021713899 hasConceptScore W3021713899C185592680 @default.
- W3021713899 hasConceptScore W3021713899C2780206646 @default.
- W3021713899 hasConceptScore W3021713899C2780437316 @default.
- W3021713899 hasConceptScore W3021713899C523546767 @default.
- W3021713899 hasConceptScore W3021713899C54355233 @default.
- W3021713899 hasConceptScore W3021713899C55493867 @default.
- W3021713899 hasConceptScore W3021713899C86803240 @default.
- W3021713899 hasConceptScore W3021713899C89423630 @default.
- W3021713899 hasIssue "1" @default.
- W3021713899 hasLocation W30217138991 @default.
- W3021713899 hasOpenAccess W3021713899 @default.
- W3021713899 hasPrimaryLocation W30217138991 @default.
- W3021713899 hasRelatedWork W1848318316 @default.
- W3021713899 hasRelatedWork W1986032595 @default.
- W3021713899 hasRelatedWork W1988017860 @default.
- W3021713899 hasRelatedWork W2002638149 @default.
- W3021713899 hasRelatedWork W2012880028 @default.
- W3021713899 hasRelatedWork W2029014577 @default.
- W3021713899 hasRelatedWork W2134675141 @default.
- W3021713899 hasRelatedWork W2364053702 @default.
- W3021713899 hasRelatedWork W242877035 @default.
- W3021713899 hasRelatedWork W3114938950 @default.
- W3021713899 hasVolume "128" @default.
- W3021713899 isParatext "false" @default.
- W3021713899 isRetracted "false" @default.
- W3021713899 magId "3021713899" @default.
- W3021713899 workType "article" @default.