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- W3021961331 abstract "The purpose of this chapter is to describe these tools and how they are used to clone and manipulate DNA constructs. First, we will discuss methods of isolating DNA fragments from the genome and other DNA vectors. Then we will describe molecular cloning: how these isolated fragments are inserted into storage vectors and mass produced using bacteria. Next, we describe how DNA fragments or recombinant DNA constructs are purified from other DNA molecules. Finally, we describe methods of reading the molecular sequence of a DNA construct. After creating and purifying a novel DNA construct, a scientist can deliver this construct into various cell types (Chapter 11) or use the construct to produce a transgenic or knockout animal (Chapters 12 and 13Chapter 12Chapter 13)." @default.
- W3021961331 created "2020-05-13" @default.
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- W3021961331 date "2015-01-01" @default.
- W3021961331 modified "2023-10-01" @default.
- W3021961331 title "Molecular Cloning and Recombinant DNA Technology" @default.
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- W3021961331 doi "https://doi.org/10.1016/b978-0-12-800511-8.00010-1" @default.
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