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- W3022124284 abstract "To construct an expression plasmid harbouring fused ovine IL 2 and D.nodosus Pilin gene,the ovine IL 2 gene with synthesized primers with EcoRⅠ and BamHⅠ recognition sequence at 5′ and 3′ end respectively was amplified and cloned from the peripheral blood of ovine.Total ovine RNA was extracted from ovine blood by Reagent TRIZOL method.Ovine IL 2 gene was amplified with ovine RNA as template by RT PCR and PCR.The amplified fragment was subjected to restriction digest and cloned into the pBluescript SK(+) vector.Then a recombinant plasmid named pBlue IL 2 was constructed.Sequence analysis confirmed that the amplified IL 2 gene is identical to the reported ovine IL 2 gene." @default.
- W3022124284 created "2020-05-13" @default.
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- W3022124284 date "2000-01-01" @default.
- W3022124284 modified "2023-09-25" @default.
- W3022124284 title "Cloning and Sequencing of Ovine Interleukin 2 Gene" @default.
- W3022124284 hasPublicationYear "2000" @default.
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