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- W3022136586 abstract "A “fusion phage” is a filamentous virion, which displays a foreign peptide fused to a coat protein and harbors the gene for the fusion protein within its genome. This chapter discusses the basic method of fusion-phage technology and describes an application for which these surface expression vectors are particularly well suited—the construction of epitope libraries. In such a library, the phages display “random” foreign peptides encoded by degenerate synthetic oligonucleotides spliced into the coat protein gene, the library as a whole representing up to billions of peptide sequences. If a phage displays a peptide that is a strong ligand for an antibody or other binding protein, it can be readily purified out of a library, even one containing a vast excess of nonbinding clones. This chapter further describes complex libraries, in which the displayed ligands are whole-folded domains. A recombinant coat protein gene can be transplanted from the phage genome into a type of plasmid called a phagemid. Phagemids contain the filamentous phage intergenic region comprising the origins of plus- and minus-strand synthesis and all other cis-acting elements needed for the synthesis of ssDNA and packaging it into virions." @default.
- W3022136586 created "2020-05-13" @default.
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- W3022136586 date "1995-01-01" @default.
- W3022136586 modified "2023-09-27" @default.
- W3022136586 title "Libraries of Peptides and Proteins Displayed on Filamentous Phage" @default.
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- W3022136586 doi "https://doi.org/10.1016/b978-0-12-765561-1.50028-x" @default.
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