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- W3022256452 abstract "Publisher SummaryThis chapter discusses that the explosion of information on transcription factors in recent years has arisen primarily because of the availability of new or improved methods to study these factors. Initially, such studies would focus on identifying a factor that interacts with a particular DNA sequence and characterizing this interaction and the methods to do this. The chapter describes a number of methods that allow the investigation of the interaction of a transcription factor with DNA, its purification, gene cloning, and dissection of its functional domains, as well as the identification of its DNA-binding site and its target genes. The chapter discusses the information obtained by the application of these procedures to particular factors. Following the identification of such a sequence will define the protein factors that bind it. This can be readily achieved by the DNA mobility shift or gel retardation assay." @default.
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- W3022256452 date "2004-01-01" @default.
- W3022256452 modified "2023-09-24" @default.
- W3022256452 title "Methods for Studying Transcription Factors" @default.
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- W3022256452 doi "https://doi.org/10.1016/b978-012437178-1/50008-4" @default.
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