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- W3022269415 abstract "Publisher SummaryThis chapter provides an overview of hemocytometer counting. Enumeration of cells propagated in vitro may be conveniently determined by using well-dispersed cell or nuclei suspensions in a standard hemocytometer chamber. In the method described in the chapter, thick glass chambers are divided into sections of calibrated area and depth. The total number of cells in a suspension is then easily calculated from the counts of cells in the hemocytometer chamber. For the preparation and counting of cell suspensions, cell monolayers are gently dispersed with enzyme and re-suspended in tissue culture medium. If the resulting cell suspension is too concentrated, a dilution should be made prior to filling the hemocytometer with the sample. For the ease and accuracy of counting, chambers should be filled with cell suspensions containing approximately 30–45 cells/mm2 on the ruled hemocytometer chamber. Dilutions may be made in a test tube or in a blood dilution pipette. If determination of the viability of cells is desired, a vital stain may be incorporated into the diluting fluid allowing simultaneous enumeration of total cells and percentage of viable cells in the suspension." @default.
- W3022269415 created "2020-05-13" @default.
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- W3022269415 date "1973-01-01" @default.
- W3022269415 modified "2023-09-26" @default.
- W3022269415 title "Hemocytometer Counting" @default.
- W3022269415 cites W1775749144 @default.
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- W3022269415 doi "https://doi.org/10.1016/b978-0-12-427150-0.50098-x" @default.
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