Matches in SemOpenAlex for { <https://semopenalex.org/work/W3022274400> ?p ?o ?g. }
- W3022274400 endingPage "3200" @default.
- W3022274400 startingPage "3200" @default.
- W3022274400 abstract "Deinococcus radiodurans is a polyextremophilic bacterium well known for its extreme resistance to irradiation, oxidative stress, and other damaging conditions. Many small noncoding RNAs (ncRNAs) in D. radiodurans have been identified by deep sequencing analysis and computational predictions. However, the precise roles of ncRNAs and their target genes in the oxidative stress response have not been investigated. Here, we report the identification and characterization of a novel ncRNA named OsiR (for oxidative stress-induced ncRNA). Oxidative stress tolerance analysis showed that deleting osiR significantly decreased viability, total antioxidant capacity, and catalase activity in D. radiodurans under oxidative stress conditions. Comparative phenotypic and qRT-PCR analyses of an osiR mutant identify a role of OsiR in regulating the expression of the catalase gene katE2. Microscale thermophoresis and genetic complementation showed that a 21-nt sequence in the stem–loop structure of OsiR (204–244 nt) directly base pairs with its counterpart in the coding region of katE2 mRNA (843–866 nt) via a 19 nt region. In addition, deletion of katE2 caused a significant reduction of catalase activity and oxidative stress tolerance similar to that observed in an osiR mutant. Our results show that OsiR positively regulates oxidative stress tolerance in D. radiodurans by increasing the mRNA stability and translation efficiency of katE2. This work provides a new regulatory pathway mediated by ncRNA for the oxidative stress response that most likely contributes to the extreme tolerances of D. radiodurans." @default.
- W3022274400 created "2020-05-13" @default.
- W3022274400 creator A5003183088 @default.
- W3022274400 creator A5038669556 @default.
- W3022274400 creator A5044864562 @default.
- W3022274400 creator A5050228668 @default.
- W3022274400 creator A5052646625 @default.
- W3022274400 creator A5065482772 @default.
- W3022274400 creator A5067916151 @default.
- W3022274400 creator A5076699095 @default.
- W3022274400 creator A5086431279 @default.
- W3022274400 date "2020-04-30" @default.
- W3022274400 modified "2023-10-16" @default.
- W3022274400 title "The Novel ncRNA OsiR Positively Regulates Expression of katE2 and is Required for Oxidative Stress Tolerance in Deinococcus radiodurans" @default.
- W3022274400 cites W1937273107 @default.
- W3022274400 cites W1979723665 @default.
- W3022274400 cites W1991095439 @default.
- W3022274400 cites W2005447726 @default.
- W3022274400 cites W2021378955 @default.
- W3022274400 cites W2031758284 @default.
- W3022274400 cites W2038219941 @default.
- W3022274400 cites W2040426430 @default.
- W3022274400 cites W2040951800 @default.
- W3022274400 cites W2044250782 @default.
- W3022274400 cites W2044770352 @default.
- W3022274400 cites W2046374424 @default.
- W3022274400 cites W2058964632 @default.
- W3022274400 cites W2066369165 @default.
- W3022274400 cites W2076105729 @default.
- W3022274400 cites W2077929618 @default.
- W3022274400 cites W2089708720 @default.
- W3022274400 cites W2090272156 @default.
- W3022274400 cites W2098508116 @default.
- W3022274400 cites W2099925408 @default.
- W3022274400 cites W2102472842 @default.
- W3022274400 cites W2111272410 @default.
- W3022274400 cites W2119463761 @default.
- W3022274400 cites W2124765964 @default.
- W3022274400 cites W2131209803 @default.
- W3022274400 cites W2135244921 @default.
- W3022274400 cites W2139271133 @default.
- W3022274400 cites W2139751207 @default.
- W3022274400 cites W2151007096 @default.
- W3022274400 cites W2155858493 @default.
- W3022274400 cites W2158757371 @default.
- W3022274400 cites W2163094428 @default.
- W3022274400 cites W2167362772 @default.
- W3022274400 cites W2192836170 @default.
- W3022274400 cites W2253793262 @default.
- W3022274400 cites W2340491446 @default.
- W3022274400 cites W2397790962 @default.
- W3022274400 cites W2405181606 @default.
- W3022274400 cites W2470099788 @default.
- W3022274400 cites W2562700014 @default.
- W3022274400 cites W2599925393 @default.
- W3022274400 cites W2624759482 @default.
- W3022274400 cites W2745945309 @default.
- W3022274400 cites W2766908207 @default.
- W3022274400 cites W2773306624 @default.
- W3022274400 cites W2897540875 @default.
- W3022274400 cites W2913682882 @default.
- W3022274400 cites W2954385716 @default.
- W3022274400 cites W2979625372 @default.
- W3022274400 doi "https://doi.org/10.3390/ijms21093200" @default.
- W3022274400 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7247583" @default.
- W3022274400 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32366051" @default.
- W3022274400 hasPublicationYear "2020" @default.
- W3022274400 type Work @default.
- W3022274400 sameAs 3022274400 @default.
- W3022274400 citedByCount "10" @default.
- W3022274400 countsByYear W30222744002020 @default.
- W3022274400 countsByYear W30222744002021 @default.
- W3022274400 countsByYear W30222744002022 @default.
- W3022274400 countsByYear W30222744002023 @default.
- W3022274400 crossrefType "journal-article" @default.
- W3022274400 hasAuthorship W3022274400A5003183088 @default.
- W3022274400 hasAuthorship W3022274400A5038669556 @default.
- W3022274400 hasAuthorship W3022274400A5044864562 @default.
- W3022274400 hasAuthorship W3022274400A5050228668 @default.
- W3022274400 hasAuthorship W3022274400A5052646625 @default.
- W3022274400 hasAuthorship W3022274400A5065482772 @default.
- W3022274400 hasAuthorship W3022274400A5067916151 @default.
- W3022274400 hasAuthorship W3022274400A5076699095 @default.
- W3022274400 hasAuthorship W3022274400A5086431279 @default.
- W3022274400 hasBestOaLocation W30222744001 @default.
- W3022274400 hasConcept C104317684 @default.
- W3022274400 hasConcept C134935766 @default.
- W3022274400 hasConcept C2776151105 @default.
- W3022274400 hasConcept C2776519032 @default.
- W3022274400 hasConcept C2778979269 @default.
- W3022274400 hasConcept C54355233 @default.
- W3022274400 hasConcept C55493867 @default.
- W3022274400 hasConcept C86803240 @default.
- W3022274400 hasConceptScore W3022274400C104317684 @default.
- W3022274400 hasConceptScore W3022274400C134935766 @default.
- W3022274400 hasConceptScore W3022274400C2776151105 @default.
- W3022274400 hasConceptScore W3022274400C2776519032 @default.
- W3022274400 hasConceptScore W3022274400C2778979269 @default.