Matches in SemOpenAlex for { <https://semopenalex.org/work/W3022277769> ?p ?o ?g. }
- W3022277769 abstract "Pancreatic islets secrete insulin from β cells and glucagon from α cells, and dysregulated secretion of these hormones is a central component of diabetes. Thus, an improved understanding of the pathways governing coordinated β and α cell hormone secretion will provide insight into islet dysfunction in diabetes. However, the 3D multicellular islet architecture, essential for coordinated islet function, presents experimental challenges for mechanistic studies of intracellular signaling pathways in primary islet cells. Here, we developed an integrated approach to study the function of primary human islet cells using genetically modified pseudoislets that resemble native islets across multiple parameters. Further, we developed a microperifusion system that allowed synchronous acquisition of GCaMP6f biosensor signal and hormone secretory profiles. We demonstrate the utility of this experimental approach by studying the effects of Gi and Gq GPCR pathways on insulin and glucagon secretion by expressing the designer receptors exclusively activated by designer drugs (DREADDs) hM4Di or hM3Dq. Activation of Gi signaling reduced insulin and glucagon secretion, while activation of Gq signaling stimulated glucagon secretion but had both stimulatory and inhibitory effects on insulin secretion, which occur through changes in intracellular Ca2+. The experimental approach of combining pseudoislets with a microfluidic system allowed the coregistration of intracellular signaling dynamics and hormone secretion and demonstrated differences in GPCR signaling pathways between human β and α cells." @default.
- W3022277769 created "2020-05-13" @default.
- W3022277769 creator A5005079621 @default.
- W3022277769 creator A5006140692 @default.
- W3022277769 creator A5014236190 @default.
- W3022277769 creator A5018991320 @default.
- W3022277769 creator A5024852088 @default.
- W3022277769 creator A5028983796 @default.
- W3022277769 creator A5034463667 @default.
- W3022277769 creator A5035136927 @default.
- W3022277769 creator A5041613431 @default.
- W3022277769 creator A5043352559 @default.
- W3022277769 creator A5049078993 @default.
- W3022277769 creator A5051320789 @default.
- W3022277769 creator A5053200897 @default.
- W3022277769 creator A5064768454 @default.
- W3022277769 creator A5084009864 @default.
- W3022277769 date "2020-05-21" @default.
- W3022277769 modified "2023-10-18" @default.
- W3022277769 title "Integrated human pseudoislet system and microfluidic platform demonstrate differences in GPCR signaling in islet cells" @default.
- W3022277769 cites W1490005573 @default.
- W3022277769 cites W1744003960 @default.
- W3022277769 cites W1925269365 @default.
- W3022277769 cites W1966403223 @default.
- W3022277769 cites W2012515461 @default.
- W3022277769 cites W2013949233 @default.
- W3022277769 cites W2015528355 @default.
- W3022277769 cites W2021920528 @default.
- W3022277769 cites W2024631759 @default.
- W3022277769 cites W2032216234 @default.
- W3022277769 cites W2061574308 @default.
- W3022277769 cites W2063116391 @default.
- W3022277769 cites W2073769248 @default.
- W3022277769 cites W2074528335 @default.
- W3022277769 cites W2086837194 @default.
- W3022277769 cites W2088251460 @default.
- W3022277769 cites W2094177994 @default.
- W3022277769 cites W2100032374 @default.
- W3022277769 cites W2106467765 @default.
- W3022277769 cites W2110468437 @default.
- W3022277769 cites W2113278846 @default.
- W3022277769 cites W2114992052 @default.
- W3022277769 cites W2142228286 @default.
- W3022277769 cites W2142418958 @default.
- W3022277769 cites W2146561547 @default.
- W3022277769 cites W2160639244 @default.
- W3022277769 cites W2171756360 @default.
- W3022277769 cites W2275148408 @default.
- W3022277769 cites W2343369957 @default.
- W3022277769 cites W2346764160 @default.
- W3022277769 cites W2413686208 @default.
- W3022277769 cites W2471853642 @default.
- W3022277769 cites W2581348935 @default.
- W3022277769 cites W2610797963 @default.
- W3022277769 cites W2733324404 @default.
- W3022277769 cites W2745223669 @default.
- W3022277769 cites W2746379904 @default.
- W3022277769 cites W2753001672 @default.
- W3022277769 cites W2755172706 @default.
- W3022277769 cites W2765153686 @default.
- W3022277769 cites W2765996430 @default.
- W3022277769 cites W2789611122 @default.
- W3022277769 cites W2792395170 @default.
- W3022277769 cites W2796003794 @default.
- W3022277769 cites W2802569467 @default.
- W3022277769 cites W2809303314 @default.
- W3022277769 cites W2810239357 @default.
- W3022277769 cites W2888657962 @default.
- W3022277769 cites W2889818981 @default.
- W3022277769 cites W2892013369 @default.
- W3022277769 cites W2899339394 @default.
- W3022277769 cites W2901208726 @default.
- W3022277769 cites W2902254549 @default.
- W3022277769 cites W2914141586 @default.
- W3022277769 cites W2914432255 @default.
- W3022277769 cites W2940492200 @default.
- W3022277769 cites W2949137252 @default.
- W3022277769 cites W2989645149 @default.
- W3022277769 doi "https://doi.org/10.1172/jci.insight.137017" @default.
- W3022277769 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7259531" @default.
- W3022277769 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32352931" @default.
- W3022277769 hasPublicationYear "2020" @default.
- W3022277769 type Work @default.
- W3022277769 sameAs 3022277769 @default.
- W3022277769 citedByCount "32" @default.
- W3022277769 countsByYear W30222777692020 @default.
- W3022277769 countsByYear W30222777692021 @default.
- W3022277769 countsByYear W30222777692022 @default.
- W3022277769 countsByYear W30222777692023 @default.
- W3022277769 crossrefType "journal-article" @default.
- W3022277769 hasAuthorship W3022277769A5005079621 @default.
- W3022277769 hasAuthorship W3022277769A5006140692 @default.
- W3022277769 hasAuthorship W3022277769A5014236190 @default.
- W3022277769 hasAuthorship W3022277769A5018991320 @default.
- W3022277769 hasAuthorship W3022277769A5024852088 @default.
- W3022277769 hasAuthorship W3022277769A5028983796 @default.
- W3022277769 hasAuthorship W3022277769A5034463667 @default.
- W3022277769 hasAuthorship W3022277769A5035136927 @default.
- W3022277769 hasAuthorship W3022277769A5041613431 @default.
- W3022277769 hasAuthorship W3022277769A5043352559 @default.