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- W3022298889 abstract "Publisher SummaryThis chapter describes the use of pronase in tissue culture. Pronase, an enzyme preparation from Streptomyces griseus, consists of a mixture of neutral and alkaline proteases, aminopeptidases, and carboxypeptidases. It has been used to remove the zona pellucida of mammalian eggs, to liberate chondrocytes from cartilage prior to freezing, and in tissue culture. Applications of Pronase in tissue culture include the dissociation of chicken and mouse embryo cells prior to cultivation and subcultivation. Pronase acts much more rapidly than trypsin, and unlike trypsin, it yields cell suspensions without large clumps of cells. It is also superior to trypsin in digesting dead cells and cellular debris. When Pronase is used to subculture cell monolayers obtained from mouse embryo cells, primary cultures of human diploid fibroblasts, and epithelial cells derived from monkey kidney tissue the cells are brought rapidly into suspension. However, certain continuous cell lines, for example, HeLa and KB are removed in flakes, indicating incomplete dispersion. The chapter describes the procedure for making primary cell cultures from 14-day mouse." @default.
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- W3022298889 date "1973-01-01" @default.
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- W3022298889 title "Pronase" @default.
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- W3022298889 doi "https://doi.org/10.1016/b978-0-12-427150-0.50007-3" @default.
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