FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3022320324> ?p ?o ?g. }

• W3022320324 abstract "Abstract Coronaviruses that cause severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) are speculated to have originated in bats. The mechanisms by which these viruses are maintained in individuals or populations of reservoir bats remain an enigma. Mathematical models have predicted long-term persistent infection with low levels of periodic shedding as a likely route for virus maintenance and spillover from bats. In this study, we tested the hypothesis that bat cells and MERS coronavirus (CoV) can co-exist in vitro . To test our hypothesis, we established a long-term coronavirus infection model of bat cells that are persistently infected with MERS-CoV. We infected cells from Eptesicus fuscus with MERS-CoV and maintained them in culture for at least 126 days. We characterized the persistently infected cells by detecting virus particles, protein and transcripts. Basal levels of type I interferon in the long-term infected bat cells were higher, relative to uninfected cells, and disrupting the interferon response in persistently infected bat cells increased virus replication. By sequencing the whole genome of MERS-CoV from persistently infected bat cells, we identified that bat cells repeatedly selected for viral variants that contained mutations in the viral open reading frame 5 (ORF5) protein. Furthermore, bat cells that were persistently infected with ΔORF5 MERS-CoV were resistant to superinfection by wildtype virus, likely due to reduced levels of the virus receptor, dipeptidyl peptidase 4 (DPP4) and higher basal levels of interferon in these cells. In summary, our study provides evidence for a model of coronavirus persistence in bats, along with the establishment of a unique persistently infected cell culture model to study MERS-CoV-bat interactions." @default.
• W3022320324 created "2020-05-13" @default.
• W3022320324 creator A5018540324 @default.
• W3022320324 creator A5041967571 @default.
• W3022320324 creator A5050567215 @default.
• W3022320324 creator A5056700979 @default.
• W3022320324 creator A5057373263 @default.
• W3022320324 creator A5058590589 @default.
• W3022320324 creator A5090457616 @default.
• W3022320324 date "2020-04-29" @default.
• W3022320324 modified "2023-09-30" @default.
• W3022320324 title "Selection of viral variants during persistent infection of insectivorous bat cells with Middle East respiratory syndrome coronavirus" @default.
• W3022320324 cites W1485276491 @default.
• W3022320324 cites W1721371055 @default.
• W3022320324 cites W1927596245 @default.
• W3022320324 cites W1964317280 @default.
• W3022320324 cites W1967828043 @default.
• W3022320324 cites W1973757205 @default.
• W3022320324 cites W1976462769 @default.
• W3022320324 cites W1989069389 @default.
• W3022320324 cites W1993577573 @default.
• W3022320324 cites W1996909392 @default.
• W3022320324 cites W2039689649 @default.
• W3022320324 cites W2042546405 @default.
• W3022320324 cites W2046153984 @default.
• W3022320324 cites W2053503974 @default.
• W3022320324 cites W2082878774 @default.
• W3022320324 cites W2089077517 @default.
• W3022320324 cites W2090139119 @default.
• W3022320324 cites W2091991657 @default.
• W3022320324 cites W2101661393 @default.
• W3022320324 cites W2101953995 @default.
• W3022320324 cites W2103441770 @default.
• W3022320324 cites W2107026529 @default.
• W3022320324 cites W2108204675 @default.
• W3022320324 cites W2117789476 @default.
• W3022320324 cites W2123702495 @default.
• W3022320324 cites W2131369206 @default.
• W3022320324 cites W2131988685 @default.
• W3022320324 cites W2140015075 @default.
• W3022320324 cites W2141802263 @default.
• W3022320324 cites W2143250568 @default.
• W3022320324 cites W2143861034 @default.
• W3022320324 cites W2149508011 @default.
• W3022320324 cites W2152770371 @default.
• W3022320324 cites W2161361622 @default.
• W3022320324 cites W2166376600 @default.
• W3022320324 cites W2166867592 @default.
• W3022320324 cites W2168576346 @default.
• W3022320324 cites W2191876267 @default.
• W3022320324 cites W2220565019 @default.
• W3022320324 cites W2269582224 @default.
• W3022320324 cites W2323806654 @default.
• W3022320324 cites W2419477968 @default.
• W3022320324 cites W2465777822 @default.
• W3022320324 cites W2467443826 @default.
• W3022320324 cites W2470646526 @default.
• W3022320324 cites W2512962334 @default.
• W3022320324 cites W2519536574 @default.
• W3022320324 cites W2559337285 @default.
• W3022320324 cites W2605364764 @default.
• W3022320324 cites W2614500237 @default.
• W3022320324 cites W2618653913 @default.
• W3022320324 cites W2626112069 @default.
• W3022320324 cites W2745861413 @default.
• W3022320324 cites W2747597014 @default.
• W3022320324 cites W2767499855 @default.
• W3022320324 cites W2777993583 @default.
• W3022320324 cites W2787479149 @default.
• W3022320324 cites W2794573360 @default.
• W3022320324 cites W2802259674 @default.
• W3022320324 cites W2886159405 @default.
• W3022320324 cites W2888750846 @default.
• W3022320324 cites W2895801917 @default.
• W3022320324 cites W2900126499 @default.
• W3022320324 cites W2913613802 @default.
• W3022320324 cites W2915545530 @default.
• W3022320324 cites W2945847460 @default.
• W3022320324 cites W4206712614 @default.
• W3022320324 doi "https://doi.org/10.1038/s41598-020-64264-1" @default.
• W3022320324 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7190632" @default.
• W3022320324 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32350357" @default.
• W3022320324 hasPublicationYear "2020" @default.
• W3022320324 type Work @default.
• W3022320324 sameAs 3022320324 @default.
• W3022320324 citedByCount "21" @default.
• W3022320324 countsByYear W30223203242020 @default.
• W3022320324 countsByYear W30223203242021 @default.
• W3022320324 countsByYear W30223203242022 @default.
• W3022320324 countsByYear W30223203242023 @default.
• W3022320324 crossrefType "journal-article" @default.
• W3022320324 hasAuthorship W3022320324A5018540324 @default.
• W3022320324 hasAuthorship W3022320324A5041967571 @default.
• W3022320324 hasAuthorship W3022320324A5050567215 @default.
• W3022320324 hasAuthorship W3022320324A5056700979 @default.
• W3022320324 hasAuthorship W3022320324A5057373263 @default.
• W3022320324 hasAuthorship W3022320324A5058590589 @default.
• W3022320324 hasAuthorship W3022320324A5090457616 @default.
• W3022320324 hasBestOaLocation W30223203241 @default.
• W3022320324 hasConcept C134018914 @default.

• next