Matches in SemOpenAlex for { <https://semopenalex.org/work/W3022380156> ?p ?o ?g. }
- W3022380156 endingPage "443" @default.
- W3022380156 startingPage "443" @default.
- W3022380156 abstract "Biotin receptors are overexpressed by various types of solid cancer cells and play a significant role in tumor metabolism, growth, and metastasis. Thus, targeting the biotin receptors on tumor cells may enhance the efficiency and reduce the side-effects of chemotherapy. The aim of this study was to develop a biotin-coupled poly(amido)amine (PAMAM) (PG4.5) dendrimer nanoparticle to enhance the tumor-specific delivery and intracellular uptake of anticancer drugs via receptor-mediated endocytosis. We modified PG4.5 with diethylenetriamine (DETA) followed by biotin via an amide bond and characterized the resulting PG4.5-DETA-biotin nanoparticles by 1H NMR, FTIR, and Raman spectroscopy. Loading and releasing of gemcitabine (GEM) from PG4.5-DETA-biotin were evaluated by UV–Visible spectrophotometry. Cell viability and cellular uptake were examined by MTT assay and flow cytometry to assess the biocompatibility, cellular internalization efficiency and antiproliferative activity of PG4.5-DETA-biotin/GEM. Gemcitabine-loaded PG4.5-DETA-biotin nanoparticles were spherical with a particle size of 81.6 ± 6.08 nm and zeta potential of 0.47 ± 1.25 mV. Maximum drug-loading content and encapsulation efficiency were 10.84 ± 0.16% and 47.01 ± 0.71%, respectively. Nearly 60.54 ± 1.99% and 73.96 ± 1.14% of gemcitabine was released from PG4.5-DETA-biotin/GEM nanoparticles after 48 h at the acidic pH values of 6.5 and 5, respectively. Flow cytometry and fluorescence microscopy of cellular uptake results revealed PG4.5-DETA-biotin/GEM nanoparticles selectively targeted cancer cells in vitro. Cytotoxicity assays demonstrated gemcitabine-loaded PG4.5-DETA-biotin significantly reduced cell viability and induced apoptosis in HeLa cells. Thus, biotin-coupled PG4.5-DETA nanocarrier could provide an effective, targeted drug delivery system and selectively convey gemcitabine into tumor cells." @default.
- W3022380156 created "2020-05-13" @default.
- W3022380156 creator A5007295903 @default.
- W3022380156 creator A5050178206 @default.
- W3022380156 creator A5054692522 @default.
- W3022380156 creator A5064299123 @default.
- W3022380156 creator A5064708975 @default.
- W3022380156 creator A5082710417 @default.
- W3022380156 creator A5084108442 @default.
- W3022380156 creator A5086541695 @default.
- W3022380156 creator A5087400327 @default.
- W3022380156 creator A5088248741 @default.
- W3022380156 date "2020-05-11" @default.
- W3022380156 modified "2023-10-01" @default.
- W3022380156 title "Biotin-Decorated PAMAM G4.5 Dendrimer Nanoparticles to Enhance the Delivery, Anti-Proliferative, and Apoptotic Effects of Chemotherapeutic Drug in Cancer Cells" @default.
- W3022380156 cites W1846575105 @default.
- W3022380156 cites W1894195601 @default.
- W3022380156 cites W1933439941 @default.
- W3022380156 cites W1967393143 @default.
- W3022380156 cites W1983466982 @default.
- W3022380156 cites W2009749738 @default.
- W3022380156 cites W2016273831 @default.
- W3022380156 cites W2029583431 @default.
- W3022380156 cites W2038955456 @default.
- W3022380156 cites W2053809294 @default.
- W3022380156 cites W2069676654 @default.
- W3022380156 cites W2077813812 @default.
- W3022380156 cites W2098158472 @default.
- W3022380156 cites W2104698662 @default.
- W3022380156 cites W2129090112 @default.
- W3022380156 cites W2132198153 @default.
- W3022380156 cites W2136910649 @default.
- W3022380156 cites W2138694786 @default.
- W3022380156 cites W2156440836 @default.
- W3022380156 cites W2169769978 @default.
- W3022380156 cites W2209620742 @default.
- W3022380156 cites W2224450631 @default.
- W3022380156 cites W2225218318 @default.
- W3022380156 cites W2275576764 @default.
- W3022380156 cites W2320478770 @default.
- W3022380156 cites W2321534064 @default.
- W3022380156 cites W2321810168 @default.
- W3022380156 cites W2370624763 @default.
- W3022380156 cites W2529227495 @default.
- W3022380156 cites W2568331132 @default.
- W3022380156 cites W2748359040 @default.
- W3022380156 cites W2761493287 @default.
- W3022380156 cites W2780433383 @default.
- W3022380156 cites W2782175804 @default.
- W3022380156 cites W2783133589 @default.
- W3022380156 cites W2788080481 @default.
- W3022380156 cites W2788206402 @default.
- W3022380156 cites W2792407585 @default.
- W3022380156 cites W2792971737 @default.
- W3022380156 cites W2794263726 @default.
- W3022380156 cites W2805168616 @default.
- W3022380156 cites W2885395493 @default.
- W3022380156 cites W2887355579 @default.
- W3022380156 cites W2908143346 @default.
- W3022380156 cites W2919201825 @default.
- W3022380156 cites W2955838941 @default.
- W3022380156 cites W2971938640 @default.
- W3022380156 cites W2975151552 @default.
- W3022380156 cites W2978032853 @default.
- W3022380156 cites W2979636644 @default.
- W3022380156 cites W2983838904 @default.
- W3022380156 cites W2987465679 @default.
- W3022380156 cites W2997396315 @default.
- W3022380156 doi "https://doi.org/10.3390/pharmaceutics12050443" @default.
- W3022380156 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7284937" @default.
- W3022380156 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32403321" @default.
- W3022380156 hasPublicationYear "2020" @default.
- W3022380156 type Work @default.
- W3022380156 sameAs 3022380156 @default.
- W3022380156 citedByCount "27" @default.
- W3022380156 countsByYear W30223801562020 @default.
- W3022380156 countsByYear W30223801562021 @default.
- W3022380156 countsByYear W30223801562022 @default.
- W3022380156 countsByYear W30223801562023 @default.
- W3022380156 crossrefType "journal-article" @default.
- W3022380156 hasAuthorship W3022380156A5007295903 @default.
- W3022380156 hasAuthorship W3022380156A5050178206 @default.
- W3022380156 hasAuthorship W3022380156A5054692522 @default.
- W3022380156 hasAuthorship W3022380156A5064299123 @default.
- W3022380156 hasAuthorship W3022380156A5064708975 @default.
- W3022380156 hasAuthorship W3022380156A5082710417 @default.
- W3022380156 hasAuthorship W3022380156A5084108442 @default.
- W3022380156 hasAuthorship W3022380156A5086541695 @default.
- W3022380156 hasAuthorship W3022380156A5087400327 @default.
- W3022380156 hasAuthorship W3022380156A5088248741 @default.
- W3022380156 hasBestOaLocation W30223801561 @default.
- W3022380156 hasConcept C109316439 @default.
- W3022380156 hasConcept C121608353 @default.
- W3022380156 hasConcept C12554922 @default.
- W3022380156 hasConcept C126322002 @default.
- W3022380156 hasConcept C153911025 @default.
- W3022380156 hasConcept C178790620 @default.
- W3022380156 hasConcept C185592680 @default.