Matches in SemOpenAlex for { <https://semopenalex.org/work/W30226079> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W30226079 endingPage "60" @default.
- W30226079 startingPage "357" @default.
- W30226079 abstract "Stabile hybridoma cells, colonies of hybridoma cells 14 days after fusion of immune spleen and myeloma cells, myeloma cells and fibroblasts cultured in 96-well microculture plates were frozen by the method of two-step freezing. The culture medium was aspirated, and 50 microliter of the medium containing a cryoprotectant (5% dimethyl sulphoxide) was added for 10 min at room temperature. The plates were put into microtene bags, placed at -25 degrees C in a freezer for 30 min and then stored at -100 degrees C in liquid nitrogen vapour. Plates with cells were thawed rapidly in a 50 degree C water bath. After thawing the hybrid cells were viable and continued to produce the specific antibody." @default.
- W30226079 created "2016-06-24" @default.
- W30226079 creator A5003790467 @default.
- W30226079 creator A5050677369 @default.
- W30226079 date "1985-01-01" @default.
- W30226079 modified "2023-09-23" @default.
- W30226079 title "Two-step freezing of hybridoma cells in 96-well microculture plates." @default.
- W30226079 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4065377" @default.
- W30226079 hasPublicationYear "1985" @default.
- W30226079 type Work @default.
- W30226079 sameAs 30226079 @default.
- W30226079 citedByCount "1" @default.
- W30226079 crossrefType "journal-article" @default.
- W30226079 hasAuthorship W30226079A5003790467 @default.
- W30226079 hasAuthorship W30226079A5050677369 @default.
- W30226079 hasConcept C108939769 @default.
- W30226079 hasConcept C153911025 @default.
- W30226079 hasConcept C178790620 @default.
- W30226079 hasConcept C179933525 @default.
- W30226079 hasConcept C185592680 @default.
- W30226079 hasConcept C196843134 @default.
- W30226079 hasConcept C202751555 @default.
- W30226079 hasConcept C203014093 @default.
- W30226079 hasConcept C2780931953 @default.
- W30226079 hasConcept C43617362 @default.
- W30226079 hasConcept C49377226 @default.
- W30226079 hasConcept C55493867 @default.
- W30226079 hasConcept C68528507 @default.
- W30226079 hasConcept C86803240 @default.
- W30226079 hasConcept C95444343 @default.
- W30226079 hasConceptScore W30226079C108939769 @default.
- W30226079 hasConceptScore W30226079C153911025 @default.
- W30226079 hasConceptScore W30226079C178790620 @default.
- W30226079 hasConceptScore W30226079C179933525 @default.
- W30226079 hasConceptScore W30226079C185592680 @default.
- W30226079 hasConceptScore W30226079C196843134 @default.
- W30226079 hasConceptScore W30226079C202751555 @default.
- W30226079 hasConceptScore W30226079C203014093 @default.
- W30226079 hasConceptScore W30226079C2780931953 @default.
- W30226079 hasConceptScore W30226079C43617362 @default.
- W30226079 hasConceptScore W30226079C49377226 @default.
- W30226079 hasConceptScore W30226079C55493867 @default.
- W30226079 hasConceptScore W30226079C68528507 @default.
- W30226079 hasConceptScore W30226079C86803240 @default.
- W30226079 hasConceptScore W30226079C95444343 @default.
- W30226079 hasIssue "5" @default.
- W30226079 hasLocation W302260791 @default.
- W30226079 hasOpenAccess W30226079 @default.
- W30226079 hasPrimaryLocation W302260791 @default.
- W30226079 hasRelatedWork W1480621434 @default.
- W30226079 hasRelatedWork W1483795044 @default.
- W30226079 hasRelatedWork W1581388243 @default.
- W30226079 hasRelatedWork W1964937715 @default.
- W30226079 hasRelatedWork W2129223841 @default.
- W30226079 hasRelatedWork W2157929710 @default.
- W30226079 hasRelatedWork W2378278214 @default.
- W30226079 hasRelatedWork W2411351693 @default.
- W30226079 hasRelatedWork W30226079 @default.
- W30226079 hasRelatedWork W3139684246 @default.
- W30226079 hasVolume "31" @default.
- W30226079 isParatext "false" @default.
- W30226079 isRetracted "false" @default.
- W30226079 magId "30226079" @default.
- W30226079 workType "article" @default.