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- W3022664601 abstract "Efforts are underway in a number of laboratories around the world to develop methods for the improvement of Lactobacillus strains by recombinant DNA techniques. Research has centered on: (i) characterization and construction of chimeric shuttle vectors based on endogenous Lactobacillus plasmids which are capable of replicating in lactobacilli; (ii) molecular cloning of genes and operons from lactobacilli encoding important pathways such as the lactose : phosphoenolpyruvate phosphotransferase system, phosphogalactoside-β-d-galactohydrolase, and β-galactosidase; and (iii) methods for introduction of genes in vivo and in vitro through conjugation, transfection and transformation. The lack of natural gene exchange systems has prompted research efforts to devise a protoplast transformation system. Initial early successes in gene cloning, vector development, transfection, conjugation, protoplast fusion and, recently, transformation, have laid the ground-work for rapid development of gene exchange systems for the genus." @default.
- W3022664601 created "2020-05-13" @default.
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- W3022664601 date "1987-09-01" @default.
- W3022664601 modified "2023-09-23" @default.
- W3022664601 title "Prospects for the genetic manipulation of lactobacilli" @default.
- W3022664601 doi "https://doi.org/10.1016/0378-1097(87)90114-5" @default.
- W3022664601 hasPublicationYear "1987" @default.
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