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• W3022698926 abstract "Macaw palm is a highly oil-producing plant, which presents high contents of free fatty acids, being a promising feedstock for biofuel production. The current chemical routes are costly and complex, involving highly harsh industrial conditions. Enzymatic processing is a potential alternative; however, it is hampered by the scarce knowledge on biocatalysts adapted to this acidic feedstock. This work describes a novel lipase isolated from the thermophilic fungus Rasamsonia emersonii (ReLip), which tolerates extreme conditions such as the presence of methanol, high temperatures and acidic medium. Among the tested feedstocks, the enzyme showed the highest preference for macaw palm oil, producing a hydrolysate with a final free fatty acid content of 92%. Crystallographic studies revealed a closed conformation of the helical amphipathic lid that typically undergoes conformational changes in a mechanism of interfacial activation. Such conformation of the lid is stabilized by a salt bridge, not observed in other structurally characterized homologues, which is likely involved in the tolerance to organic solvents. Moreover, the lack of conservation of the aromatic cluster IxxWxxxxxF in the lid of ReLip with the natural mutation of the phenylalanine by an alanine might be correlated with the preference of short acyl chains, although preserving catalytic activity on insoluble substrates. In addition, the presence of five acidic amino acids in the lid of ReLip, a rare property reported in other lipases, may have contributed to its ability to tolerate and be effective in acidic environments. Therefore, our work describes a new fungal biocatalyst capable of efficiently hydrolyzing macaw oil, an attractive feedstock for the production of “drop-in” biofuels, with high desirable feature for industrial conditions such as thermal and methanol tolerance, and optimum acidic pH. Moreover, the crystallographic structure was elucidated, providing a structural basis for the enzyme substrate preference and tolerance to organic solvents." @default.
• W3022698926 created "2020-05-13" @default.
• W3022698926 creator A5002784629 @default.
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• W3022698926 creator A5082381783 @default.
• W3022698926 date "2020-05-06" @default.
• W3022698926 modified "2023-10-04" @default.
• W3022698926 title "A Novel Fungal Lipase With Methanol Tolerance and Preference for Macaw Palm Oil" @default.
• W3022698926 cites W1636124898 @default.
• W3022698926 cites W1964340580 @default.
• W3022698926 cites W1964556874 @default.
• W3022698926 cites W1970414304 @default.
• W3022698926 cites W1975421696 @default.
• W3022698926 cites W1976806277 @default.
• W3022698926 cites W1990902952 @default.
• W3022698926 cites W1995201241 @default.
• W3022698926 cites W2000493953 @default.
• W3022698926 cites W2001783660 @default.
• W3022698926 cites W2002189289 @default.
• W3022698926 cites W2005622335 @default.
• W3022698926 cites W2009010596 @default.
• W3022698926 cites W2011810297 @default.
• W3022698926 cites W2012618268 @default.
• W3022698926 cites W2012682314 @default.
• W3022698926 cites W2014031151 @default.
• W3022698926 cites W2018618159 @default.
• W3022698926 cites W2020928799 @default.
• W3022698926 cites W2021689495 @default.
• W3022698926 cites W2028806666 @default.
• W3022698926 cites W2029408863 @default.
• W3022698926 cites W2036714804 @default.
• W3022698926 cites W2038960066 @default.
• W3022698926 cites W2041007873 @default.
• W3022698926 cites W2043622844 @default.
• W3022698926 cites W2046820889 @default.
• W3022698926 cites W2047748350 @default.
• W3022698926 cites W2056033583 @default.
• W3022698926 cites W2057047871 @default.
• W3022698926 cites W2061750017 @default.
• W3022698926 cites W2064100699 @default.
• W3022698926 cites W2067431380 @default.
• W3022698926 cites W2069156111 @default.
• W3022698926 cites W2070173743 @default.
• W3022698926 cites W2072674961 @default.
• W3022698926 cites W2073480927 @default.
• W3022698926 cites W2073683847 @default.
• W3022698926 cites W2081367949 @default.
• W3022698926 cites W2082431727 @default.
• W3022698926 cites W2082759569 @default.
• W3022698926 cites W2083598389 @default.
• W3022698926 cites W2084432213 @default.
• W3022698926 cites W2093635147 @default.
• W3022698926 cites W2100074804 @default.
• W3022698926 cites W2102304943 @default.
• W3022698926 cites W2107164874 @default.
• W3022698926 cites W2118383750 @default.
• W3022698926 cites W2119734896 @default.
• W3022698926 cites W2121531429 @default.
• W3022698926 cites W2122994276 @default.
• W3022698926 cites W2129683862 @default.
• W3022698926 cites W2130543846 @default.
• W3022698926 cites W2135661937 @default.
• W3022698926 cites W2147658175 @default.
• W3022698926 cites W2149885731 @default.
• W3022698926 cites W2180229411 @default.
• W3022698926 cites W2184948893 @default.
• W3022698926 cites W2320310848 @default.
• W3022698926 cites W2331441635 @default.
• W3022698926 cites W2402061767 @default.
• W3022698926 cites W2422495325 @default.
• W3022698926 cites W2461762400 @default.
• W3022698926 cites W2492118049 @default.
• W3022698926 cites W2511329964 @default.
• W3022698926 cites W2535454580 @default.
• W3022698926 cites W2561296282 @default.
• W3022698926 cites W2565824544 @default.
• W3022698926 cites W2584903755 @default.
• W3022698926 cites W2591967910 @default.
• W3022698926 cites W2606562059 @default.
• W3022698926 cites W2619559076 @default.
• W3022698926 cites W2752554449 @default.
• W3022698926 cites W2753434565 @default.
• W3022698926 cites W2787810748 @default.
• W3022698926 cites W2791482593 @default.
• W3022698926 cites W2888685803 @default.
• W3022698926 cites W2898474497 @default.
• W3022698926 cites W2912682093 @default.
• W3022698926 cites W2913381691 @default.
• W3022698926 cites W2918167638 @default.
• W3022698926 cites W2921314423 @default.
• W3022698926 cites W4210675630 @default.
• W3022698926 cites W4248872320 @default.

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