FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3022716035> ?p ?o ?g. }

• W3022716035 endingPage "102469" @default.
• W3022716035 startingPage "102469" @default.
• W3022716035 abstract "Previous studies have reported robust inflammatory cell infiltration, synthesis of IgG, B-cell clonal expansion, deposition of immune complexes and complement within cerebral cavernous malformation (CCM) lesions. B-cell depletion has also been shown to reduce the maturation of CCM in murine models. We hypothesize that antigen(s) within the lesional milieu perpetuate the pathogenetic immune responses in CCMs. This study aims to identify those putative antigen(s) using monoclonal antibodies (mAbs) derived from plasma cells found in surgically removed human CCM lesions. We produced human mAbs from laser capture micro-dissected plasma cells from four CCM patients, and also germline-reverted versions. CCM mAbs were assayed using immunofluorescence on central nervous system (CNS) tissues and immunocytochemistry on human primary cell lines. Antigen characterization was performed using a combination of confocal microscopy, immunoprecipitation and mass spectrometry. Affinity was determined by enzyme-linked immunosorbent assay, and specificity by multi-color confocal microscopy and quantitative co-localization. CCM mAbs bound CNS tissue, especially endothelial cells and astrocytes. Non-muscle myosin heavy chain IIA (NMMHCIIA), vimentin and tubulin are three cytoskeleton proteins that were commonly targeted. Selection of cytoskeleton proteins by plasma cells was supported by a high frequency of immunoglobulin variable region somatic hypermutations, high affinity and selectivity of mAbs in their affinity matured forms, and profoundly reduced affinity and selectivity in the germline reverted forms. Antibodies produced by plasma cells in CCM lesions commonly target cytoplasmic and cytoskeletal autoantigens including NMMHCIIA, vimentin and tubulin that are abundant in endothelial cells and astrocytes. Binding to, and selection on autoantigen(s) in the lesional milieu likely perpetuates the pathogenetic immune response in CCMs. Blocking this in situ autoimmune response may yield a novel treatment for CCM." @default.
• W3022716035 created "2020-05-13" @default.
• W3022716035 creator A5002141375 @default.
• W3022716035 creator A5007880392 @default.
• W3022716035 creator A5010076935 @default.
• W3022716035 creator A5027395645 @default.
• W3022716035 creator A5039582994 @default.
• W3022716035 creator A5050082012 @default.
• W3022716035 creator A5060397504 @default.
• W3022716035 creator A5061354710 @default.
• W3022716035 creator A5061444906 @default.
• W3022716035 creator A5072016469 @default.
• W3022716035 creator A5079621916 @default.
• W3022716035 creator A5082214506 @default.
• W3022716035 creator A5084407986 @default.
• W3022716035 creator A5087790193 @default.
• W3022716035 creator A5090963814 @default.
• W3022716035 date "2020-09-01" @default.
• W3022716035 modified "2023-10-17" @default.
• W3022716035 title "Antibodies in cerebral cavernous malformations react with cytoskeleton autoantigens in the lesional milieu" @default.
• W3022716035 cites W101344820 @default.
• W3022716035 cites W1563271400 @default.
• W3022716035 cites W1807019775 @default.
• W3022716035 cites W1938025088 @default.
• W3022716035 cites W1974024509 @default.
• W3022716035 cites W1975548815 @default.
• W3022716035 cites W1977732091 @default.
• W3022716035 cites W1996626942 @default.
• W3022716035 cites W2005141785 @default.
• W3022716035 cites W2011667781 @default.
• W3022716035 cites W2021446933 @default.
• W3022716035 cites W2021990477 @default.
• W3022716035 cites W2025139875 @default.
• W3022716035 cites W2043020898 @default.
• W3022716035 cites W2043270774 @default.
• W3022716035 cites W2065734531 @default.
• W3022716035 cites W2066922712 @default.
• W3022716035 cites W2073235331 @default.
• W3022716035 cites W2074626057 @default.
• W3022716035 cites W2075460902 @default.
• W3022716035 cites W2093977796 @default.
• W3022716035 cites W2110537012 @default.
• W3022716035 cites W2116051694 @default.
• W3022716035 cites W2123082276 @default.
• W3022716035 cites W2124294512 @default.
• W3022716035 cites W2134080501 @default.
• W3022716035 cites W2163209710 @default.
• W3022716035 cites W2171538469 @default.
• W3022716035 cites W2340078952 @default.
• W3022716035 cites W2461036323 @default.
• W3022716035 cites W2602923846 @default.
• W3022716035 cites W2749915319 @default.
• W3022716035 cites W2763973319 @default.
• W3022716035 cites W2802101579 @default.
• W3022716035 cites W2809683128 @default.
• W3022716035 cites W2810549935 @default.
• W3022716035 cites W2824322894 @default.
• W3022716035 cites W2889362907 @default.
• W3022716035 cites W2889835308 @default.
• W3022716035 cites W2892334779 @default.
• W3022716035 cites W2893720134 @default.
• W3022716035 cites W2954759368 @default.
• W3022716035 cites W2969804619 @default.
• W3022716035 cites W4238285434 @default.
• W3022716035 doi "https://doi.org/10.1016/j.jaut.2020.102469" @default.
• W3022716035 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7483292" @default.
• W3022716035 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32362501" @default.
• W3022716035 hasPublicationYear "2020" @default.
• W3022716035 type Work @default.
• W3022716035 sameAs 3022716035 @default.
• W3022716035 citedByCount "3" @default.
• W3022716035 countsByYear W30227160352022 @default.
• W3022716035 countsByYear W30227160352023 @default.
• W3022716035 crossrefType "journal-article" @default.
• W3022716035 hasAuthorship W3022716035A5002141375 @default.
• W3022716035 hasAuthorship W3022716035A5007880392 @default.
• W3022716035 hasAuthorship W3022716035A5010076935 @default.
• W3022716035 hasAuthorship W3022716035A5027395645 @default.
• W3022716035 hasAuthorship W3022716035A5039582994 @default.
• W3022716035 hasAuthorship W3022716035A5050082012 @default.
• W3022716035 hasAuthorship W3022716035A5060397504 @default.
• W3022716035 hasAuthorship W3022716035A5061354710 @default.
• W3022716035 hasAuthorship W3022716035A5061444906 @default.
• W3022716035 hasAuthorship W3022716035A5072016469 @default.
• W3022716035 hasAuthorship W3022716035A5079621916 @default.
• W3022716035 hasAuthorship W3022716035A5082214506 @default.
• W3022716035 hasAuthorship W3022716035A5084407986 @default.
• W3022716035 hasAuthorship W3022716035A5087790193 @default.
• W3022716035 hasAuthorship W3022716035A5090963814 @default.
• W3022716035 hasBestOaLocation W30227160352 @default.
• W3022716035 hasConcept C134018914 @default.
• W3022716035 hasConcept C142669718 @default.
• W3022716035 hasConcept C147447768 @default.
• W3022716035 hasConcept C147483822 @default.
• W3022716035 hasConcept C1491633281 @default.
• W3022716035 hasConcept C153911025 @default.
• W3022716035 hasConcept C159654299 @default.
• W3022716035 hasConcept C203014093 @default.

• next