FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3022725209> ?p ?o ?g. }

• W3022725209 endingPage "577" @default.
• W3022725209 startingPage "569" @default.
• W3022725209 abstract "Aggregation of the TAU proteins in the form of neurofibrillary tangles (NFTs) in the brain is a common risk factor in tauopathies including Alzheimer's disease (AD). Several strategies have been implemented to target NFTs, among which chaperones, which facilitate the proper folding of proteins, appear to hold great promise in effectively inhibiting TAU polymerization. The aim of this study was to analyze the impact of the chaperone Artemin on TAU aggregation in vitro.In this experimental study, recombinant TAU- or Artemin proteins were expressed in E.coli bacteria, and purified using ion-exchange and affinity chromatography. Sodium dodecyl sulfate-poly acrylamide gel electrophoresis (SDS-PAGE) was used to run the extracted proteins and check their purity. Heparin was used as an aggregation inducer. The interaction kinetics of TAU aggregation and disassembly was performed using thioflavin T (ThT) fluorescence analysis and circular dichroism (CD) spectroscopy.Ion-exchange and affinity chromatography yielded highly pure TAU and Artemin proteins for subsequent analyses. In addition, we found that heparin efficiently induced TAU fibrillization 48 hours post-incubation, as evidenced by ThT assay. Importantly, Artemin was observed to effectively block the aggregation of both physiologic- and supraphysiologic TAU concentrations in a dose-dependent manner, as judged by ThT and CD spectroscopy analyses.Our collective results show, for the first time, that the chaperone Artemin could significantly inhibit aggregation of the TAU proteins in a dose-dependent manner, and support Artemin as a potential potent blocker of TAU aggregation in people with AD." @default.
• W3022725209 created "2020-05-13" @default.
• W3022725209 creator A5020989304 @default.
• W3022725209 creator A5046421644 @default.
• W3022725209 creator A5052992732 @default.
• W3022725209 creator A5088667038 @default.
• W3022725209 creator A5091054291 @default.
• W3022725209 date "2018-01-01" @default.
• W3022725209 modified "2023-10-16" @default.
• W3022725209 title "The Molecular Chaperone Artemin Efficiently Blocks Fibrillization of TAU Protein In Vitro." @default.
• W3022725209 cites W1518158761 @default.
• W3022725209 cites W1530520327 @default.
• W3022725209 cites W1551057808 @default.
• W3022725209 cites W1974305786 @default.
• W3022725209 cites W1978278862 @default.
• W3022725209 cites W1985002125 @default.
• W3022725209 cites W1990473479 @default.
• W3022725209 cites W1996199105 @default.
• W3022725209 cites W2002559713 @default.
• W3022725209 cites W2005628434 @default.
• W3022725209 cites W2007746236 @default.
• W3022725209 cites W2016162029 @default.
• W3022725209 cites W2021919788 @default.
• W3022725209 cites W2023707229 @default.
• W3022725209 cites W2026892692 @default.
• W3022725209 cites W2034286717 @default.
• W3022725209 cites W2035920420 @default.
• W3022725209 cites W2038107727 @default.
• W3022725209 cites W2042485050 @default.
• W3022725209 cites W2043931405 @default.
• W3022725209 cites W2046436217 @default.
• W3022725209 cites W2049771698 @default.
• W3022725209 cites W2057850942 @default.
• W3022725209 cites W2068668571 @default.
• W3022725209 cites W2070017758 @default.
• W3022725209 cites W2071875670 @default.
• W3022725209 cites W2075421841 @default.
• W3022725209 cites W2078770779 @default.
• W3022725209 cites W2089475153 @default.
• W3022725209 cites W2091893114 @default.
• W3022725209 cites W2092075823 @default.
• W3022725209 cites W2099795098 @default.
• W3022725209 cites W2099991048 @default.
• W3022725209 cites W2101152249 @default.
• W3022725209 cites W2110544463 @default.
• W3022725209 cites W2112169034 @default.
• W3022725209 cites W2112360486 @default.
• W3022725209 cites W2122522567 @default.
• W3022725209 cites W2126128897 @default.
• W3022725209 cites W2137140827 @default.
• W3022725209 cites W2163859059 @default.
• W3022725209 cites W2165525275 @default.
• W3022725209 cites W2302116106 @default.
• W3022725209 cites W2309256909 @default.
• W3022725209 cites W2310154553 @default.
• W3022725209 cites W2319574713 @default.
• W3022725209 cites W2410912226 @default.
• W3022725209 cites W4210975612 @default.
• W3022725209 cites W4211135065 @default.
• W3022725209 doi "https://doi.org/10.22074/cellj.2018.4510" @default.
• W3022725209 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5672095" @default.
• W3022725209 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29105391" @default.
• W3022725209 hasPublicationYear "2018" @default.
• W3022725209 type Work @default.
• W3022725209 sameAs 3022725209 @default.
• W3022725209 citedByCount "3" @default.
• W3022725209 countsByYear W30227252092020 @default.
• W3022725209 countsByYear W30227252092021 @default.
• W3022725209 crossrefType "journal-article" @default.
• W3022725209 hasAuthorship W3022725209A5020989304 @default.
• W3022725209 hasAuthorship W3022725209A5046421644 @default.
• W3022725209 hasAuthorship W3022725209A5052992732 @default.
• W3022725209 hasAuthorship W3022725209A5088667038 @default.
• W3022725209 hasAuthorship W3022725209A5091054291 @default.
• W3022725209 hasConcept C116084860 @default.
• W3022725209 hasConcept C12554922 @default.
• W3022725209 hasConcept C133571119 @default.
• W3022725209 hasConcept C136238340 @default.
• W3022725209 hasConcept C142724271 @default.
• W3022725209 hasConcept C185592680 @default.
• W3022725209 hasConcept C202751555 @default.
• W3022725209 hasConcept C204328495 @default.
• W3022725209 hasConcept C2775962898 @default.
• W3022725209 hasConcept C2778896982 @default.
• W3022725209 hasConcept C2778913249 @default.
• W3022725209 hasConcept C2779134260 @default.
• W3022725209 hasConcept C502032728 @default.
• W3022725209 hasConcept C55493867 @default.
• W3022725209 hasConcept C71924100 @default.
• W3022725209 hasConcept C86803240 @default.
• W3022725209 hasConceptScore W3022725209C116084860 @default.
• W3022725209 hasConceptScore W3022725209C12554922 @default.
• W3022725209 hasConceptScore W3022725209C133571119 @default.
• W3022725209 hasConceptScore W3022725209C136238340 @default.
• W3022725209 hasConceptScore W3022725209C142724271 @default.
• W3022725209 hasConceptScore W3022725209C185592680 @default.
• W3022725209 hasConceptScore W3022725209C202751555 @default.
• W3022725209 hasConceptScore W3022725209C204328495 @default.

• next