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• W3022753624 abstract "The working hypothesis that steroid hormones are transferred into the nucleus of the target cells in combination with a protein receptor and there alter the transcription of one or more portions of the genome is supported by experimental data from many laboratories. There are difficulties in identifying and measuring the immediate product of the transcription process, mRNA, and none of the several criteria used to distinguish messenger from ribosomal and transfer RNA has been completely satisfactory. The messenger RNA's of eukaryotic cells appear to contain a covalently linked region of 100 or more nucleotides rich in polyriboadenylic acid linked to the mRNA at the 3′-OH end. The injection of estradiol into an adult castrate rat increases the total amount of uterine poly (A)-rich RNA and the effectiveness of this material in inducing enzyme synthesis when instilled into the uterine lumen. RNA extracted from the uterus of an estrogen treated rat and instilled into the uterine lumen of an immature rat increases protein synthesis and the activities of glucose-6-phosphate dehydrogenase and ornithine decarboxylase. The effective RNA, a very small fraction of the total, is separable from the mass of RNA by chromatography on a sepharose 4B polyuridylic acid column. This suggests that the RNA effective when instilled in utero has a poly-adenylic acid portion and may be a messenger RNA. The RNA not bound to poly U sepharose is without effect in stimulating protein synthesis or in increasing the activity of G-6-PDH or ODC when instilled in utero." @default.
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• W3022753624 date "1975-01-01" @default.
• W3022753624 modified "2023-09-26" @default.
• W3022753624 title "EFFECTS OF ESTROGENS ON UTERINE POLY (A)-RICH RNA" @default.
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• W3022753624 doi "https://doi.org/10.1016/b978-0-08-019709-8.50025-3" @default.
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