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• W3022969804 abstract "We delineate a label free amperometric immunosensor for the diagnosis of lung cancer by detecting CD59, which is a clinically relevant biomarker of the lung cancer. The working assembly of the immuno-sensor was achieved via immobilization of the anti-CD59 antibodies on a graphene oxide nanoparticles (GrONPs) decorated pencil graphite electrode. The GrONPs were applied to improve the conductivity of the immunosensor. The characterization of probe fabrication was carried out using the scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). In order to attain the maximum activity, the experimental parameters were optimized in the terms of antibody concentration, pH, incubation time & temperature and antigen concentration. The detection of CD59 was accomplished by measuring the current, which revealed a range between 1 fgmL−1 to 10 ngmL−1. The disclosed minimum limit of detection (LOD) of the sensor was 1 fgmL−1 . The presence of CD59 was also investigated in first morning urine samples of lung cancer patients and compared that of the apparently healthy adults using the immuno-sensor. The potential of immunosensor was evident by its good storage stability and specificity." @default.
• W3022969804 created "2020-05-13" @default.
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• W3022969804 date "2020-07-01" @default.
• W3022969804 modified "2023-10-18" @default.
• W3022969804 title "An electrochemical CD59 targeted noninvasive immunosensor based on graphene oxide nanoparticles embodied pencil graphite for detection of lung cancer" @default.
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• W3022969804 doi "https://doi.org/10.1016/j.microc.2020.104957" @default.
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