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• W3023006585 abstract "To investigate the mechanism of p38 mitogen activated protein kinase (MAPK) signaling pathway in regulating the hyperplasia and hypertrophy of human lumbar ligamentum flavum via transforming growth factor β 1 (TGF-β 1)/connective tissue growth factor (CTGF).The lumbar ligamentum flavum tissue taken from patient with lumbar intervertebral disc herniation was isolated by collagenase-predigested explant cultures. The ligamentum flavum cells were treated with the extracellular regulated protein kinase pathway blocker PD98059, c-Jun N-terminal kinase pathway blocker SP600125, and p38 pathway blocker SB203580, and then the mRNA expressions of CTGF, collagen type Ⅰ, and collagen type Ⅲ were detected by real-time fluorescence quantitative PCR (qRT-PCR). The ligamentum flavum cells were divided into 4 groups, and transfected with small interfering RNA (siRNA), p38 siRNA, siRNA+3 ng/mL TGF-β 1, and p38 siRNA+3 ng/mL TGF-β 1 in groups A, B, C, and D, respectively. After 24 hours of transfection, immunofluorescence staining was performed to observe the expressions of p38 and phosphorylation p38 (p-p38); the relative mRNA expressions of CTGF, collagen type Ⅰ, and collagen type Ⅲ in each group were detected by qRT-PCR; the protein expression of CTGF in each group was detected by Western blot.p38 pathway blocker SB203580 could significantly reduce the relative mRNA expressions of CTGF, collagen type Ⅰ, and collagen type Ⅲ ( P<0.05). After 24 hours of transfection, immunofluorescence staining showed positive staining with p38 and p-p38 expressions in groups A, C, and D and negative staining in group B. Compared with group A, the relative mRNA expressions of CTGF, collagen type Ⅰ, and collagen type Ⅲ and relative protein expression of CTGF in group B decreased significantly ( P<0.05), while those in groups C and D increased significantly ( P<0.05); and those indicators significantly increased in group C than in group D ( P<0.05).TGF-β 1/CTGF based on the p38 MAPK signaling pathway play an important role in the occurance and development of hypertrophy of human lumbar ligamentum flavum.探讨 p38 丝裂原活化蛋白激酶（mitogen activated protein kinase，MAPK）通路在 TGF-β 1/结缔组织生长因子（connective tissue growth factor，CTGF）调控人腰椎黄韧带增生肥厚中的作用机制。.取腰椎间盘突出髓核摘除术中获得的黄韧带组织，采用胶原酶预消化组织块培养法分离培养黄韧带细胞。分别用细胞外调节蛋白激酶通路阻断剂 PD98059、c-Jun 氨基末端激酶通路阻断剂 SP600125、p38 通路阻断剂 SB203580 处理黄韧带细胞，实时荧光定量PCR（real-time fluorescence quantitative PCR，qRT-PCR）检测 CTGF、Ⅰ型胶原和Ⅲ型胶原 mRNA 相对表达量。然后取黄韧带细胞分为 A、B、C、D 组，分别以小干扰 RNA（small interfering RNA，siRNA）、p38 siRNA、siRNA+3 ng/mL TGF-β 1、p38 siRNA+3 ng/mL TGF-β 1 转染细胞，转染 24 h 后行免疫荧光染色观察 p38 和磷酸化 p38（phosphorylation p38，p-p38）表达，qRT-PCR 检测各组 CTGF、Ⅰ型胶原和Ⅲ型胶原 mRNA 相对表达量，Western blot 检测各组 CTGF 蛋白表达。.p38 通路阻断剂 SB203580 可明显降低黄韧带细胞 CTGF、Ⅰ型胶原和Ⅲ型胶原 mRNA 相对表达量（ P<0.05）。转染 24 h 后，免疫荧光染色显示 A、C、D 组细胞呈阳性反应，有 p38、p-p38 表达，且 C、D 组强于 A 组；B 组细胞呈阴性反应，无 p38、p-p38 表达。与 A 组相比，B 组 CTGF、Ⅰ型胶原和Ⅲ型胶原 mRNA 相对表达量以及 CTGF 蛋白相对表达量显著减少，C、D 组显著增加，C 组较 D 组进一步增加，差异均有统计学意义（ P<0.05）。.p38 MAPK 通路介导 TGF-β 1/CTGF 表达，在人腰椎黄韧带细胞增生肥厚过程中具有重要作用。." @default.
• W3023006585 created "2020-05-13" @default.
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• W3023006585 date "2019-06-15" @default.
• W3023006585 modified "2023-09-23" @default.
• W3023006585 title "[Mechanism of p38 mitogen activated protein kinase signaling pathway on promoting the hypertrophy of human lumbar ligamentum flavum via transforming growth factor β 1/connective tissue growth factor]." @default.
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• W3023006585 doi "https://doi.org/10.7507/1002-1892.201811140" @default.
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