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• W3023082785 abstract "Abstract ScRNA-seq has the ability to reveal accurate and precise cell types and states. Existing scRNA-seq platforms utilize bead-based technologies uniquely barcoding individual cells, facing practical challenges for precious samples with limited cell number. Here, we present a scRNA-seq platform, named Paired-seq, with high cells/beads utilization efficiency, cell-free RNAs removal capability, high gene detection ability and low cost. We utilize the differential flow resistance principle to achieve single cell/barcoded bead pairing with high cell utilization efficiency (95%). The integration of valves and pumps enables the complete removal of cell-free RNAs, efficient cell lysis and mRNA capture, achieving highest mRNA detection accuracy (R = 0.955) and comparable sensitivity. Lower reaction volume and higher mRNA capture and barcoding efficiency significantly reduce the cost of reagents and sequencing. The single-cell expression profile of mES and drug treated cells reveal cell heterogeneity, demonstrating the enormous potential of Paired-seq for cell biology, developmental biology and precision medicine." @default.
• W3023082785 created "2020-05-13" @default.
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• W3023082785 date "2020-04-30" @default.
• W3023082785 modified "2023-10-15" @default.
• W3023082785 title "Highly parallel and efficient single cell mRNA sequencing with paired picoliter chambers" @default.
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• W3023082785 doi "https://doi.org/10.1038/s41467-020-15765-0" @default.
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• W3023082785 hasPublicationYear "2020" @default.
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