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• W3023171687 abstract "Introduction: IL-31 is an IL-6 type cytokine produced by activated T cells that signals via a heterodimeric receptor complex composed of IL-31Rα and OSMRβ. IL-31 plays an important pathologic role in animal models of airway hyper-reactivity and dermatitis. Other than LPS and IFNγ, the influence of inflammatory mediators on IL-31Rα expression and signaling remains largely unknown. We hypothesized that IL-31Rα expression in bronchopulmonary epithelial cells and pulmonary macrophages can be regulated by various mediators alone or in combination. Methods: IL-31Rα expression was evaluated by semi-quantitative PCR of primary cultures of human lung epithelial cells, bronchial epithelial cells, and pulmonary macrophages. IL-31Rα expression was also evaluated in HBE135 human bronchial epithelial cell line and A549 human alveolar epithelial cell line. The cells were stimulated for twenty-four hours with one or more of the following mediators: Interferon γ, Transforming Growth Factor β, IL-31, hyper IL-6, lipopolysaccharide, dexamethasone, and an inflammatory mediator mixture (MM, conditioned media derived from lipopolysaccharide activated pulmonary macrophages). IL-31Rα signaling in these epithelial cell lines was evaluated by Western blot analysis of STAT3 and ERK1/2 phosphorylation. Results: Evaluation of the effects of IL-31, hyper IL-6, and LPS treatment on A549 and HBE135 cells failed to demonstrate increased IL-31Rα expression. Evaluation of IL-31Rα signaling by Western blot analysis of A549 and HBE135 cells indicated that only pretreatment with TGFβ or dexamethasone increased IL-31 mediated STAT3 and ERK1/2 phosphorylation. Conclusions: Basal levels of the IL-31Rα transcript were detectable in epithelial cell lines but not in primary epithelial cell or pulmonary macrophage cultures. Treatment with TGFβ or dexamethasone increased IL-31Rα expression and corresponding signaling in epithelial cells. The combination of TGFβ and MM further increased IL-31Rα expression. The data suggest that in the setting of bronchopulmonary inflammation, where TGFβ and/or complex inflammatory mediator mixtures are present, IL-31Rα expression and signaling may be increased. Tabled 1IL-31Rα Expression by PCR A549 HBE135 Lung Epithelial Cells Bronchial Epithelial Cells Pulmonary Macrophages Basal + + - - - IFNγ + + - - + TGFβ ++ ++ + + - MM ++ + n/a n/a n/a TGFβ+MM +++ +++ n/a n/a n/a Dexamethasone ++ ++ +/- +/- n/a Open table in a new tab" @default.
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• W3023171687 date "2007-02-01" @default.
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• W3023171687 doi "https://doi.org/10.1016/j.jss.2006.12.335" @default.
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