FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3023193177> ?p ?o ?g. }

• W3023193177 abstract "Abstract Preimplantation genetic testing for aneuploidies (PGT-A) using trophectoderm (TE) biopsy samples is labour intensive, invasive, and subject to sampling bias. In this study, we report on the efficacy and factors affecting accuracy of a technique we pioneered for minimally invasive preimplantation genetic testing for aneuploidy (miPGT-A). Our technique uses cell-free embryonic DNA (cfeDNA) in spent embryo culture medium (SEM) combined with blastocoel fluid (BF) to increase the amount of assayable cfeDNA. We compared miPGT-A results (n = 145 embryos) with standard PGT-A analysis of the corresponding trophectoderm biopsy. We found that accuracy of miPGT was not related to blastocyst morphological grade. The overall concordance rate per sample for euploidy/aneuploidy status between miPGT-A and TE biopsy samples was 88/90 (97.8%), and was not different between good 47/48 (97.9%) and moderate/low quality blastocysts 41/42 (97.9%) (p > 0.05). Importantly, we also discovered that for cfeDNA analysis, the SurePlex whole genome amplification (WGA) kit can be utilized without an additional cell lysis/extraction DNA step; this efficiency likely reduces the risk of maternal contamination. Regarding origin of embryonic cfeDNA, the average amount of miPGT-A WGA-DNA we obtained from blastocysts with different morphological grades, as well as the size miPGT-A WGA-DNA fragments, suggest that it is unlikely that apoptosis and necrosis are only mechanisms of DNA release from the inner cell mass (ICM) and TE into BF and SEM." @default.
• W3023193177 created "2020-05-13" @default.
• W3023193177 creator A5002060988 @default.
• W3023193177 creator A5006990205 @default.
• W3023193177 creator A5010023591 @default.
• W3023193177 creator A5011244070 @default.
• W3023193177 creator A5022421146 @default.
• W3023193177 creator A5026263499 @default.
• W3023193177 creator A5039238222 @default.
• W3023193177 creator A5058055096 @default.
• W3023193177 creator A5078492450 @default.
• W3023193177 date "2020-04-29" @default.
• W3023193177 modified "2023-10-18" @default.
• W3023193177 title "Minimally Invasive Cell-Free Human Embryo Aneuploidy Testing (miPGT-A) Utilizing Combined Spent Embryo Culture Medium and Blastocoel Fluid –Towards Development of a Clinical Assay" @default.
• W3023193177 cites W1580824804 @default.
• W3023193177 cites W1604381192 @default.
• W3023193177 cites W1967639239 @default.
• W3023193177 cites W1999711244 @default.
• W3023193177 cites W2040011204 @default.
• W3023193177 cites W2102158139 @default.
• W3023193177 cites W2104006121 @default.
• W3023193177 cites W2110331794 @default.
• W3023193177 cites W2146711145 @default.
• W3023193177 cites W2164648299 @default.
• W3023193177 cites W2182179011 @default.
• W3023193177 cites W2252927984 @default.
• W3023193177 cites W2277371716 @default.
• W3023193177 cites W2282627631 @default.
• W3023193177 cites W2409176010 @default.
• W3023193177 cites W2510544585 @default.
• W3023193177 cites W2519815557 @default.
• W3023193177 cites W2523884988 @default.
• W3023193177 cites W2524720610 @default.
• W3023193177 cites W2560645298 @default.
• W3023193177 cites W2564870360 @default.
• W3023193177 cites W2603671750 @default.
• W3023193177 cites W2767784030 @default.
• W3023193177 cites W2786514708 @default.
• W3023193177 cites W2789928641 @default.
• W3023193177 cites W2795277171 @default.
• W3023193177 cites W2801510830 @default.
• W3023193177 cites W2803285616 @default.
• W3023193177 cites W2807413624 @default.
• W3023193177 cites W2808010278 @default.
• W3023193177 cites W2810219433 @default.
• W3023193177 cites W2888208687 @default.
• W3023193177 cites W2897458775 @default.
• W3023193177 cites W2897592722 @default.
• W3023193177 cites W2901190885 @default.
• W3023193177 cites W2908736683 @default.
• W3023193177 cites W2912287467 @default.
• W3023193177 cites W2919888313 @default.
• W3023193177 cites W2923235670 @default.
• W3023193177 cites W2923432472 @default.
• W3023193177 cites W2937412615 @default.
• W3023193177 cites W2943703259 @default.
• W3023193177 cites W2947820190 @default.
• W3023193177 cites W2950219943 @default.
• W3023193177 cites W2953611911 @default.
• W3023193177 cites W2955296255 @default.
• W3023193177 cites W2988798068 @default.
• W3023193177 cites W2991431315 @default.
• W3023193177 doi "https://doi.org/10.1038/s41598-020-64335-3" @default.
• W3023193177 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7190856" @default.
• W3023193177 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32350403" @default.
• W3023193177 hasPublicationYear "2020" @default.
• W3023193177 type Work @default.
• W3023193177 sameAs 3023193177 @default.
• W3023193177 citedByCount "25" @default.
• W3023193177 countsByYear W30231931772020 @default.
• W3023193177 countsByYear W30231931772021 @default.
• W3023193177 countsByYear W30231931772022 @default.
• W3023193177 countsByYear W30231931772023 @default.
• W3023193177 crossrefType "journal-article" @default.
• W3023193177 hasAuthorship W3023193177A5002060988 @default.
• W3023193177 hasAuthorship W3023193177A5006990205 @default.
• W3023193177 hasAuthorship W3023193177A5010023591 @default.
• W3023193177 hasAuthorship W3023193177A5011244070 @default.
• W3023193177 hasAuthorship W3023193177A5022421146 @default.
• W3023193177 hasAuthorship W3023193177A5026263499 @default.
• W3023193177 hasAuthorship W3023193177A5039238222 @default.
• W3023193177 hasAuthorship W3023193177A5058055096 @default.
• W3023193177 hasAuthorship W3023193177A5078492450 @default.
• W3023193177 hasBestOaLocation W30231931771 @default.
• W3023193177 hasConcept C104317684 @default.
• W3023193177 hasConcept C142724271 @default.
• W3023193177 hasConcept C159399396 @default.
• W3023193177 hasConcept C16685009 @default.
• W3023193177 hasConcept C196843134 @default.
• W3023193177 hasConcept C2775934546 @default.
• W3023193177 hasConcept C2777804106 @default.
• W3023193177 hasConcept C2778177303 @default.
• W3023193177 hasConcept C2779672484 @default.
• W3023193177 hasConcept C30481170 @default.
• W3023193177 hasConcept C54355233 @default.
• W3023193177 hasConcept C68760520 @default.
• W3023193177 hasConcept C71924100 @default.
• W3023193177 hasConcept C86803240 @default.
• W3023193177 hasConcept C87073359 @default.
• W3023193177 hasConceptScore W3023193177C104317684 @default.

• next