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• W3023291212 abstract "Abstract In recent decades, tissue engineering techniques have attracted much attention in the construction of 3D tissues or organs. However, even though precise control of cell locations in 3D has been achieved, the organized cell locations are easily destroyed because of the cell migration during the cell culture period. In human body, basement membranes (BMs) maintain the precise cell locations in 3D (compartmentalization). Constructing artificial BMs that mimic the structure and biofunctions of natural BMs remains a major challenge. Here, a nanometer‐sized artificial BM through layer‐by‐layer assembly of collagen type IV (Col‐IV) and laminin (LM), chosen because they are the main components of natural BMs, is reported. This multilayered Col‐IV/LM nanofilm imitates natural BM structure closely, showing controllable and similar components, thickness, and fibrous network. The Col‐IV/LM nanofilms have high cell adhesion properties and maintain the spreading morphology effectively. Furthermore, the barrier effect of preventing cell migration but permitting effective cell–cell crosstalk between fibroblasts and endothelial cells demonstrates the ability of Col‐IV/LM nanofilms for cell compartmentalization in 3D tissues, providing more reliable tissue models for evaluating drug efficacy, nanotoxicology, and implantation." @default.
• W3023291212 created "2020-05-13" @default.
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• W3023291212 date "2020-05-05" @default.
• W3023291212 modified "2023-10-14" @default.
• W3023291212 title "Fabrication of Artificial Nanobasement Membranes for Cell Compartmentalization in 3D Tissues" @default.
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• W3023291212 doi "https://doi.org/10.1002/smll.201907434" @default.
• W3023291212 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32372510" @default.
• W3023291212 hasPublicationYear "2020" @default.
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