FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3023404704> ?p ?o ?g. }

• W3023404704 endingPage "2782" @default.
• W3023404704 startingPage "2769" @default.
• W3023404704 abstract "Medin, a 50-amino-acid cleavage product of the milk fat globule-EGF factor 8 protein, is one of the most common forms of localized amyloid found in the vasculature of individuals older than 50 years. Medin induces endothelial dysfunction and vascular inflammation, yet despite its prevalence in the human aorta and multiple arterial beds, little is known about the nature of its pathology. Medin oligomers have been implicated in the pathology of aortic aneurysm, aortic dissection, and more recently, vascular dementia. Recent in vitro biomechanical measurements found increased oligomer levels in aneurysm patients with altered aortic wall integrity. Our results suggest an oligomer-mediated toxicity mechanism for medin pathology. Using lipid bilayer electrophysiology, we show that medin oligomers induce ionic membrane permeability by pore formation. Pore activity was primarily observed for preaggregated medin species from the growth-phase and rarely for lag-phase species. Atomic force microscopy (AFM) imaging of medin aggregates at different stages of aggregation revealed the gradual formation of flat domains resembling the morphology of supported lipid bilayers. Transmission electron microscopy images showed the coexistence of compact oligomers, largely consistent with the AFM data, and larger protofibrillar structures. Circular dichroism spectroscopy revealed the presence of largely disordered species and suggested the presence of β-sheets. This observation and the significantly lower thioflavin T fluorescence emitted by medin aggregates compared to amyloid-β fibrils, along with the absence of amyloid fibers in the AFM and transmission electron microscopy images, suggest that medin aggregation into pores follows a nonamyloidogenic pathway. In silico modeling by molecular dynamics simulations provides atomic-level structural detail of medin pores with the CNpNC barrel topology and diameters comparable to values estimated from experimental pore conductances." @default.
• W3023404704 created "2020-05-13" @default.
• W3023404704 creator A5006553652 @default.
• W3023404704 creator A5025357844 @default.
• W3023404704 creator A5041456482 @default.
• W3023404704 creator A5046394948 @default.
• W3023404704 creator A5052491243 @default.
• W3023404704 creator A5071084031 @default.
• W3023404704 creator A5078966815 @default.
• W3023404704 creator A5080433759 @default.
• W3023404704 creator A5081140590 @default.
• W3023404704 date "2020-06-01" @default.
• W3023404704 modified "2023-10-14" @default.
• W3023404704 title "Medin Oligomer Membrane Pore Formation: A Potential Mechanism of Vascular Dysfunction" @default.
• W3023404704 cites W146020132 @default.
• W3023404704 cites W1553877445 @default.
• W3023404704 cites W1602910611 @default.
• W3023404704 cites W1970736092 @default.
• W3023404704 cites W1974187431 @default.
• W3023404704 cites W1976739923 @default.
• W3023404704 cites W1977456654 @default.
• W3023404704 cites W1982959887 @default.
• W3023404704 cites W1983195484 @default.
• W3023404704 cites W1986050360 @default.
• W3023404704 cites W1989574240 @default.
• W3023404704 cites W1989810100 @default.
• W3023404704 cites W1991009079 @default.
• W3023404704 cites W1991241547 @default.
• W3023404704 cites W1991651056 @default.
• W3023404704 cites W1993028164 @default.
• W3023404704 cites W1995191747 @default.
• W3023404704 cites W1999007957 @default.
• W3023404704 cites W2000464699 @default.
• W3023404704 cites W2001830061 @default.
• W3023404704 cites W2010227280 @default.
• W3023404704 cites W2016566401 @default.
• W3023404704 cites W2020462791 @default.
• W3023404704 cites W2023603224 @default.
• W3023404704 cites W2024349223 @default.
• W3023404704 cites W2025249219 @default.
• W3023404704 cites W2026380581 @default.
• W3023404704 cites W2026981923 @default.
• W3023404704 cites W2028872675 @default.
• W3023404704 cites W2033085140 @default.
• W3023404704 cites W2033433194 @default.
• W3023404704 cites W2038091759 @default.
• W3023404704 cites W2038455600 @default.
• W3023404704 cites W2039823464 @default.
• W3023404704 cites W2047098878 @default.
• W3023404704 cites W2047577462 @default.
• W3023404704 cites W2048144762 @default.
• W3023404704 cites W2052685422 @default.
• W3023404704 cites W2057709942 @default.
• W3023404704 cites W2060914703 @default.
• W3023404704 cites W2062990728 @default.
• W3023404704 cites W2064367582 @default.
• W3023404704 cites W2065100984 @default.
• W3023404704 cites W2066414494 @default.
• W3023404704 cites W2066767743 @default.
• W3023404704 cites W2067214698 @default.
• W3023404704 cites W2073686561 @default.
• W3023404704 cites W2074619433 @default.
• W3023404704 cites W2075930760 @default.
• W3023404704 cites W2077362716 @default.
• W3023404704 cites W2080319524 @default.
• W3023404704 cites W2084414517 @default.
• W3023404704 cites W2087861584 @default.
• W3023404704 cites W2100817247 @default.
• W3023404704 cites W2103440561 @default.
• W3023404704 cites W2109713145 @default.
• W3023404704 cites W2114200547 @default.
• W3023404704 cites W2121342475 @default.
• W3023404704 cites W2121977115 @default.
• W3023404704 cites W2126606962 @default.
• W3023404704 cites W2129583700 @default.
• W3023404704 cites W2131819352 @default.
• W3023404704 cites W2132113235 @default.
• W3023404704 cites W2134909516 @default.
• W3023404704 cites W2142253858 @default.
• W3023404704 cites W2144535585 @default.
• W3023404704 cites W2149081087 @default.
• W3023404704 cites W2150981663 @default.
• W3023404704 cites W2162166182 @default.
• W3023404704 cites W2165186407 @default.
• W3023404704 cites W2171132114 @default.
• W3023404704 cites W2187470614 @default.
• W3023404704 cites W2313952418 @default.
• W3023404704 cites W2329808372 @default.
• W3023404704 cites W2560790409 @default.
• W3023404704 cites W2597780090 @default.
• W3023404704 cites W2738386976 @default.
• W3023404704 cites W2763204922 @default.
• W3023404704 cites W2773490123 @default.
• W3023404704 cites W2800654508 @default.
• W3023404704 cites W2803200197 @default.
• W3023404704 cites W2808618567 @default.
• W3023404704 cites W2809078375 @default.
• W3023404704 cites W2909222904 @default.

• next