FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3023653023> ?p ?o ?g. }

• W3023653023 abstract "Author(s): Reitz, Zachary Joseph Weber | Advisor(s): Downing, Timothy L | Abstract: Cells are known to sense and respond to their mechanical microenvironment in profound ways. Various evidence has implicated the adhesome, a body of adhesion associated proteins, in several cell fate decisions, including differentiation and proliferation. Furthermore, recent work has demonstrated that substrate topography can modulate the epigenetic state of fibroblasts, facilitate cell reprogramming, and temper inflammatory activation. However, the effectors responsible for such phenomena remain poorly understood. Here we explore the effect of biomaterial design, adhesion, and intracellular forces of the epigenetic and transcriptional regulation of cell state during macrophage activation and somatic cell reprogramming.Regarding macrophage activation, we relied entirely on murine bone marrow-derived macrophages (BMDMs). To study the effect of adhesion on BMDM behavior, we utilized microcontact printing to produce fibronectin patterned substrates ontop of which we seeded BMDMs. Next, we induced macrophage activation using lipopolysaccharide (LPS) and interferon-γ (INFγ) and found patterned substrates reduce inflammatory gene expression and histone-3 acetylation (H3Ac) while also increasing cellular elongation. We incorporated these results into a bioinformatic reanalysis of transcriptional and epigenetic data derived from a study of H3Ac protein binding during macrophage polarization. This analysis allowed us to identify epigenetic mechanisms linking the adhesome and inflammatory gene expression.In our investigation of somatic cell reprogramming, we found extracellular matrix binding (ECM) and mechanosensitive ion channel activation reduce reprogramming efficiency. In addition to these results, we discovered that 104 adhesome genes are dynamically regulated during the reprogramming process. Subsequently, we performed an shRNA knockdown of each of these genes and found over 90% of our knockdowns increased the number of TRA-1-60 positive pluripotent colonies. Our knockdown of SHROOM3, a gene associated with apical cellular constriction, increased reprogramming efficiency by 27-fold. Further investigation into SHROOM3 identified a mechano-sensitive critical state transition, which may be necessary for successful reprogramming.These observations establish adhesome gene expression and mechanical signaling as influential regulators of cell fate during macrophage activation and reprogramming. Moreover, our findings may guide future attempts to regulate cell state and fate using biophysical stimuli. They may also help shed light on cell behavior in various disease states involving cancer and inflammation." @default.
• W3023653023 created "2020-05-13" @default.
• W3023653023 creator A5073098308 @default.
• W3023653023 date "2019-01-01" @default.
• W3023653023 modified "2023-09-27" @default.
• W3023653023 title "The Biophysical Micro-Environment’s Influence on Cell Fate Decisions During Macrophage Activation and Somatic Cell Reprogramming" @default.
• W3023653023 cites W1587510658 @default.
• W3023653023 cites W1665218804 @default.
• W3023653023 cites W1979069664 @default.
• W3023653023 cites W1987557442 @default.
• W3023653023 cites W1990174038 @default.
• W3023653023 cites W2003315801 @default.
• W3023653023 cites W2014677321 @default.
• W3023653023 cites W2024315189 @default.
• W3023653023 cites W2033700277 @default.
• W3023653023 cites W2036897871 @default.
• W3023653023 cites W2044939023 @default.
• W3023653023 cites W2045269599 @default.
• W3023653023 cites W2051388483 @default.
• W3023653023 cites W2060611411 @default.
• W3023653023 cites W2074744546 @default.
• W3023653023 cites W2077540512 @default.
• W3023653023 cites W2080473885 @default.
• W3023653023 cites W2091119718 @default.
• W3023653023 cites W2092076185 @default.
• W3023653023 cites W2096147603 @default.
• W3023653023 cites W2099954462 @default.
• W3023653023 cites W2115856515 @default.
• W3023653023 cites W2133879630 @default.
• W3023653023 cites W2134208433 @default.
• W3023653023 cites W2144393227 @default.
• W3023653023 cites W2146337514 @default.
• W3023653023 cites W2162166699 @default.
• W3023653023 cites W2167202160 @default.
• W3023653023 cites W2167830630 @default.
• W3023653023 cites W2180144971 @default.
• W3023653023 cites W2197106873 @default.
• W3023653023 cites W2256191215 @default.
• W3023653023 cites W2338968478 @default.
• W3023653023 cites W2537332047 @default.
• W3023653023 cites W2591765579 @default.
• W3023653023 cites W2592811885 @default.
• W3023653023 cites W2592960310 @default.
• W3023653023 cites W2602581211 @default.
• W3023653023 cites W2897232908 @default.
• W3023653023 cites W2916837967 @default.
• W3023653023 cites W2071925280 @default.
• W3023653023 hasPublicationYear "2019" @default.
• W3023653023 type Work @default.
• W3023653023 sameAs 3023653023 @default.
• W3023653023 citedByCount "0" @default.
• W3023653023 crossrefType "posted-content" @default.
• W3023653023 hasAuthorship W3023653023A5073098308 @default.
• W3023653023 hasConcept C104317684 @default.
• W3023653023 hasConcept C1491633281 @default.
• W3023653023 hasConcept C163952510 @default.
• W3023653023 hasConcept C185592680 @default.
• W3023653023 hasConcept C41091548 @default.
• W3023653023 hasConcept C54355233 @default.
• W3023653023 hasConcept C77255625 @default.
• W3023653023 hasConcept C85789140 @default.
• W3023653023 hasConcept C86339819 @default.
• W3023653023 hasConcept C86803240 @default.
• W3023653023 hasConcept C95444343 @default.
• W3023653023 hasConceptScore W3023653023C104317684 @default.
• W3023653023 hasConceptScore W3023653023C1491633281 @default.
• W3023653023 hasConceptScore W3023653023C163952510 @default.
• W3023653023 hasConceptScore W3023653023C185592680 @default.
• W3023653023 hasConceptScore W3023653023C41091548 @default.
• W3023653023 hasConceptScore W3023653023C54355233 @default.
• W3023653023 hasConceptScore W3023653023C77255625 @default.
• W3023653023 hasConceptScore W3023653023C85789140 @default.
• W3023653023 hasConceptScore W3023653023C86339819 @default.
• W3023653023 hasConceptScore W3023653023C86803240 @default.
• W3023653023 hasConceptScore W3023653023C95444343 @default.
• W3023653023 hasLocation W30236530231 @default.
• W3023653023 hasOpenAccess W3023653023 @default.
• W3023653023 hasPrimaryLocation W30236530231 @default.
• W3023653023 hasRelatedWork W1994760449 @default.
• W3023653023 hasRelatedWork W2024930891 @default.
• W3023653023 hasRelatedWork W2052492646 @default.
• W3023653023 hasRelatedWork W2053418565 @default.
• W3023653023 hasRelatedWork W2119656037 @default.
• W3023653023 hasRelatedWork W2155997555 @default.
• W3023653023 hasRelatedWork W2156809095 @default.
• W3023653023 hasRelatedWork W2345071900 @default.
• W3023653023 hasRelatedWork W2418032620 @default.
• W3023653023 hasRelatedWork W2476151009 @default.
• W3023653023 hasRelatedWork W2593168128 @default.
• W3023653023 hasRelatedWork W2753283927 @default.
• W3023653023 hasRelatedWork W2807936745 @default.
• W3023653023 hasRelatedWork W2900896770 @default.
• W3023653023 hasRelatedWork W3083073577 @default.
• W3023653023 hasRelatedWork W3087099451 @default.
• W3023653023 hasRelatedWork W3160371754 @default.
• W3023653023 hasRelatedWork W3164122702 @default.
• W3023653023 hasRelatedWork W3174255043 @default.
• W3023653023 hasRelatedWork W183451627 @default.
• W3023653023 isParatext "false" @default.
• W3023653023 isRetracted "false" @default.

• next