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• W3023767265 startingPage "90" @default.
• W3023767265 abstract "Self-assembly of proteins into well-organized proteinosomes has attracted great interest due to the potential medical and biological applications of the structures. Herein, a new concept of electrostatic assisted fabrication of proteinosomes is proposed. The self-assembly is performed by using multi-step dialysis approach, where negatively charged bovine serum albumin-poly(N-isopropylacrylamide) (BSA-PNIPAM) bioconjugate and positively charged enzyme (lysozyme or trypsin) are initially dissolved in phosphate buffer (PB) solution at a high salt concentration, and subsequently the protein solution is dialyzed against PB solutions at low salt concentrations, resulting in the formation of biofunctional proteinosomes. Transmission electron microscopy (TEM), cryo-TEM and light scattering results all demonstrate the formation of hollow structures. The wall of a proteinosome is composed of BSA and enzyme (lysozyme or trypsin), and PNIPAM chains of the bioconjugate are in the corona stabilizing the structure. In comparison with the native enzymes, the enzyme molecules in the assemblies basically retain their bioactivities. The proteinosomes formed by BSA-PNIPAM and lysozyme can be dissociated in the presence of trypsin, and those self-assembled by BSA-PNIPAM and trypsin are able to be self-hydrolyzed, resulting in the dissociation of the structures in aqueous solution. The size and morphology changes of the proteinosomes in the hydrolysis are studied." @default.
• W3023767265 created "2020-05-13" @default.
• W3023767265 creator A5007877552 @default.
• W3023767265 creator A5013543484 @default.
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• W3023767265 date "2020-09-01" @default.
• W3023767265 modified "2023-10-16" @default.
• W3023767265 title "Electrostatic assisted fabrication and dissociation of multi-component proteinosomes" @default.
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• W3023767265 doi "https://doi.org/10.1016/j.jcis.2020.05.013" @default.
• W3023767265 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32408164" @default.
• W3023767265 hasPublicationYear "2020" @default.
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