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- W3023873107 abstract "SUMMARY The in vitro germination of pollen in sorghum is useful on studies of viability, physiology, and genetic transformation of pollen. However, the reported media are low efficient. This research aims to develop an artificial medium and to determine the optimal conditions for pollen germination of sorghum. It was used a factorial arrangement of concentrations of sucrose, boric acid, and calcium nitrate; besides, it was evaluated the effect of pH, relative humidity, physical state of the media, and the floral developed stage on pollen collection. The optimal medium was done using 0.7 M of sucrose, 3.81 mM of calcium nitrate, 1.21 mM of boric acid, and 1% of agar with pH 6. The highest germination was obtained with pollen collected just after anthesis and relative humidity above to 70 %. The non germinated pollen grains was negatively correlated with relative humidity (-0.47, p>0.01). With the conditions described in this paper was obtained up to 51% of" @default.
- W3023873107 created "2020-05-13" @default.
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- W3023873107 date "2011-01-01" @default.
- W3023873107 modified "2023-09-27" @default.
- W3023873107 title "DEVELOPMENT OF AN EFFICIENT METHOD FOR in vitro GERMINATION OF SORGHUM POLLEN)" @default.
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