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- W3023932022 abstract "This chapter discusses the culturing of aspen callus. It discusses that firm white callus from aspen trees has been initiated and maintained in an actively growing condition since 1961 at The Institute of Paper Chemistry. Seven species of Populus have been cultured, as well as numerous hybrids and polyploids. One advantage of this technique is its simplicity over collecting cambial explants from mature trees. In the method described in the chapter, callus is derived from the cambium of succulent juvenile plants and is composed of large, thin-walled cells which are chemically similar to xylem-initial cells normally produced in trees. The chapter also describes the root sprout production method developed by Mathes for internode explants from rooted root sprouts. In this method, current-year branches from young trees, natural field suckers from roots, or seedlings can be used if the bark is relatively smooth. The chapter also explains that callus production may vary among explants, in regard to both the rate of growth and quality of the tissue. However, the final stock culture should be composed of uniformly firm white callus, preferably originating from one parental explant." @default.
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- W3023932022 date "1973-01-01" @default.
- W3023932022 modified "2023-09-24" @default.
- W3023932022 title "Aspen Callus" @default.
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- W3023932022 doi "https://doi.org/10.1016/b978-0-12-427150-0.50045-0" @default.
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