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• W3023984445 abstract "Metal ions play important roles in the diverse biochemical reactions associated with many cell signalling pathways. The modification of biomaterials with metal ions may offer a promising approach to stimulate cellular activity for improving tissue regeneration. Here, copper ion loading as a potential therapeutic agent in silk fibroin (SF) scaffolds was investigated. Freezing-annealing was used to induce silk I crystallization for forming water-insoluble SF scaffolds. Cu(II) ions were entrapped into SF scaffolds with different ratios by forming silk I crystal networks when copper chloride dihydrate was less than 5.0 wt%, producing water-stable materials. Moreover, it was found that copper ion chelation further enhanced SF stability when a low amount copper chloride was loaded. Increasing copper chloride content weakened silk I crystallization and Cu(II) ion chelation, rendering SF scaffolds unstable in water. Above 5.0 wt% copper chloride dihydrate, silk I crystallization was prevented. Finally, silk I scaffold with 1.5 wt% copper chloride dihydrate showed the strongest water-stability and highest loading efficiency. The results provide valuable data for understanding the effect of metal ions in freezing-induced SF crystallization, and also offer options for preparing novel Cu(II)-functionalized SF scaffolds." @default.
• W3023984445 created "2020-05-13" @default.
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• W3023984445 date "2020-09-01" @default.
• W3023984445 modified "2023-10-14" @default.
• W3023984445 title "Cu(II) ion loading in silk fibroin scaffolds with silk I structure" @default.
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• W3023984445 doi "https://doi.org/10.1016/j.ijbiomac.2020.04.094" @default.
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