Matches in SemOpenAlex for { <https://semopenalex.org/work/W3024172009> ?p ?o ?g. }
- W3024172009 endingPage "7043" @default.
- W3024172009 startingPage "7034" @default.
- W3024172009 abstract "Abstract The microvascular endothelial network is essential for bone formation and regeneration. In this context, endothelial cells not only support vascularization but also influence bone physiology via cell contact‐dependent mechanisms. In order to improve vascularization and osteogenesis in tissue engineering applications, several strategies have been developed. One promising approach is the coapplication of endothelial and adipose derived stem cells (ADSCs). In this study, we aimed at investigating the best ratio of human umbilical vein endothelial cells (HUVECs) and osteogenic differentiated ADSCs with regard to proliferation, apoptosis, osteogenesis and angiogenesis. For this purpose, cocultures of ADSCs and HUVECs with ratios of 25%:75%, 50%:50% and 75%:25% were performed. We were able to prove that cocultivation supports proliferation whereas apoptosis was unidirectional decreased in cocultured HUVECs mediated by a p‐BAD‐dependent mechanism. Moreover, coculturing ADSCs and HUVECs stimulated matrix mineralization and the activity of alkaline phosphatase (ALP). Increased gene expression of the proangiogenic markers eNOS, Flt, Ang2 and MMP3 as well as sprouting phenomena in matrigel assays proved the angiogenic potential of the coculture. In summary, coculturing ADSCs and HUVECs stimulates proliferation, cell survival, osteogenesis and angiogenesis particularly in the 50%:50% coculture." @default.
- W3024172009 created "2020-05-21" @default.
- W3024172009 creator A5008569109 @default.
- W3024172009 creator A5022916804 @default.
- W3024172009 creator A5024497698 @default.
- W3024172009 creator A5065097566 @default.
- W3024172009 creator A5073547259 @default.
- W3024172009 creator A5091023310 @default.
- W3024172009 date "2020-05-12" @default.
- W3024172009 modified "2023-10-06" @default.
- W3024172009 title "Bone tissue engineering using adipose‐derived stem cells and endothelial cells: Effects of the cell ratio" @default.
- W3024172009 cites W1573363574 @default.
- W3024172009 cites W1650896052 @default.
- W3024172009 cites W1941535010 @default.
- W3024172009 cites W1959810868 @default.
- W3024172009 cites W1963806550 @default.
- W3024172009 cites W1974081986 @default.
- W3024172009 cites W1975111290 @default.
- W3024172009 cites W1997023449 @default.
- W3024172009 cites W2001766723 @default.
- W3024172009 cites W2002702688 @default.
- W3024172009 cites W2011979365 @default.
- W3024172009 cites W2020333453 @default.
- W3024172009 cites W2024923637 @default.
- W3024172009 cites W2026575398 @default.
- W3024172009 cites W2027970223 @default.
- W3024172009 cites W2029025070 @default.
- W3024172009 cites W2030643309 @default.
- W3024172009 cites W2033203667 @default.
- W3024172009 cites W2035984463 @default.
- W3024172009 cites W2036348189 @default.
- W3024172009 cites W2036551233 @default.
- W3024172009 cites W2045047231 @default.
- W3024172009 cites W2045680273 @default.
- W3024172009 cites W2048049479 @default.
- W3024172009 cites W2063998346 @default.
- W3024172009 cites W2065460430 @default.
- W3024172009 cites W2075436227 @default.
- W3024172009 cites W2082611827 @default.
- W3024172009 cites W2086738705 @default.
- W3024172009 cites W2091608820 @default.
- W3024172009 cites W2093306419 @default.
- W3024172009 cites W2093628776 @default.
- W3024172009 cites W2097767970 @default.
- W3024172009 cites W2098526024 @default.
- W3024172009 cites W2101682146 @default.
- W3024172009 cites W2121483420 @default.
- W3024172009 cites W2127624074 @default.
- W3024172009 cites W2130362099 @default.
- W3024172009 cites W2168789685 @default.
- W3024172009 cites W2182832651 @default.
- W3024172009 cites W2238155309 @default.
- W3024172009 cites W2294754125 @default.
- W3024172009 cites W2319562462 @default.
- W3024172009 cites W2320760301 @default.
- W3024172009 cites W2341991389 @default.
- W3024172009 cites W2490899035 @default.
- W3024172009 cites W2574265596 @default.
- W3024172009 cites W2738286434 @default.
- W3024172009 cites W2795265104 @default.
- W3024172009 cites W2888378128 @default.
- W3024172009 cites W2913936227 @default.
- W3024172009 cites W2944933026 @default.
- W3024172009 cites W2945726720 @default.
- W3024172009 cites W2963218360 @default.
- W3024172009 cites W2977527692 @default.
- W3024172009 cites W615236574 @default.
- W3024172009 doi "https://doi.org/10.1111/jcmm.15374" @default.
- W3024172009 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7299704" @default.
- W3024172009 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32394620" @default.
- W3024172009 hasPublicationYear "2020" @default.
- W3024172009 type Work @default.
- W3024172009 sameAs 3024172009 @default.
- W3024172009 citedByCount "15" @default.
- W3024172009 countsByYear W30241720092020 @default.
- W3024172009 countsByYear W30241720092021 @default.
- W3024172009 countsByYear W30241720092022 @default.
- W3024172009 countsByYear W30241720092023 @default.
- W3024172009 crossrefType "journal-article" @default.
- W3024172009 hasAuthorship W3024172009A5008569109 @default.
- W3024172009 hasAuthorship W3024172009A5022916804 @default.
- W3024172009 hasAuthorship W3024172009A5024497698 @default.
- W3024172009 hasAuthorship W3024172009A5065097566 @default.
- W3024172009 hasAuthorship W3024172009A5073547259 @default.
- W3024172009 hasAuthorship W3024172009A5091023310 @default.
- W3024172009 hasBestOaLocation W30241720091 @default.
- W3024172009 hasConcept C123012128 @default.
- W3024172009 hasConcept C160160445 @default.
- W3024172009 hasConcept C171089720 @default.
- W3024172009 hasConcept C181199279 @default.
- W3024172009 hasConcept C185592680 @default.
- W3024172009 hasConcept C202751555 @default.
- W3024172009 hasConcept C203014093 @default.
- W3024172009 hasConcept C2777411675 @default.
- W3024172009 hasConcept C2778271429 @default.
- W3024172009 hasConcept C2778608917 @default.
- W3024172009 hasConcept C2780394083 @default.
- W3024172009 hasConcept C28328180 @default.