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• W3024581643 abstract "In this work, the concept of lipase cocktail has been proposed in the ultrasound-assisted hydrolysis of coconut oil. Lipase from Thermomyces lanuginosus (TLL), lipase from Rhizomucor miehei (RML), and lipase B from Candida antarctica (CALB) were evaluated as biocatalysts in different combinations. The best conversion (33.66%) was achieved using only RML; however, the best lipase cocktail (75% RML and 25% CALB) proposed by the triangular response surface was used to achieve higher conversions. At the best lipase cocktail, reaction parameters [temperature, biocatalyst content and molar ratio (water/oil)] were optimized by a Central Composite Design, allowing to obtain more than 98% of conversion in the hydrolysis of coconut oil in 3 h of incubation at 37 kHz, 300 W and 45 °C by using 20% of the lipase cocktail (w/w) and a molar ratio of 7.5:1 (water/oil). The lipase cocktail retained about 50% of its initial activity after three consecutive cycles of hydrolysis. To the authors’ knowledge, up to date, this communication is the first report in the literature for the ultrasound-assisted hydrolysis of coconut oil catalyzed by a cocktail of lipases. Under ultrasound irradiation, the concept of lipase cocktail was successfully applied, and this strategy could be useful for the other types of reactions using heterogeneous substrates." @default.
• W3024581643 created "2020-05-21" @default.
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• W3024581643 date "2020-05-15" @default.
• W3024581643 modified "2023-10-04" @default.
• W3024581643 title "Sonohydrolysis using an enzymatic cocktail in the preparation of free fatty acid" @default.
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• W3024581643 doi "https://doi.org/10.1007/s13205-020-02227-z" @default.
• W3024581643 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7229052" @default.
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