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- W3024748893 abstract "Objective The group IV chitinase from Helicoverpa armigera (HaCHT4)was expressed in prokaryotic and its polyclonal antibody against HaCHT4 was prepared. Methods The fusion protein His-HaCHT4 was expressed in E.coli and purified the target protein by Ni-column, and immunized the mice by footpad and subcutaneous injection. The titer of polyclonal antibody against His-HaCHT4 was determined by ELISA after four immunizations. The immunological specificity of the anti His-HaCHT4 was assayed by Western blot analysis. Results Fusion protein His-HaCHT4 was purified successfully and the titer of mouse anti His-HaCHT4 polyclonal antibody was higher than 1:204 800. The results of Western blot showed that the anti His-HaCHT4 polyclonal antibody was able to bind the fusion protein His-HaCHT4, and also specifically recognize the natural HaCHT4 in Helicoverpa armigera, but not the protein in Tenebrio molitor. Conclusion His-HaCHT4 was successfully expressed in prokaryotic and purified, and its mouse anti His-HaCHT4 polyclonal antibody was prepared." @default.
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- W3024748893 date "2018-09-01" @default.
- W3024748893 modified "2023-09-26" @default.
- W3024748893 title "[Preparation and identification of anti-HaCHT4 mouse polyclonal antibody]." @default.
- W3024748893 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30463659" @default.
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