Matches in SemOpenAlex for { <https://semopenalex.org/work/W3024778851> ?p ?o ?g. }
Showing items 1 to 57 of
57
with 100 items per page.
- W3024778851 endingPage "415" @default.
- W3024778851 startingPage "413" @default.
- W3024778851 abstract "A high performance liquid chromatography (HPLC) method was established for the detection of 2, 3-pyridinedicarboximide (PDI) and its enzyme reaction products, 3-carbamoyl-α-picolinic acid (α-3CP), using an engineering strain containing the D-hydantoinase gene expression box.The strain pET3a-hyd/BL21(DE3) was collected after induction and added to a PDI saturated aqueous solution.After reacting at 37℃ for 30 min with constant stirring, the supernatant was separated by centrifugation at 13000 r/min and detected by HPLC.The chromatographic conditions were as follows:HypersilTM GOLD C18 column (250 mm×4.6 mm, 5 μm), H2O-acetonitrile (90:10, v/v) containing 0.1%(v/v) trifluoroacetic acid as the mobile phase with a flow rate of 1 mL/min and a detection wavelength of 254 nm.The specific activity of pET3a-hyd/BL21(DE3) was found to be 0.61 U/(mL·10OD600 nm).This study provides a theoretical basis for the preparation of complicated half-amides using biological methods." @default.
- W3024778851 created "2020-05-21" @default.
- W3024778851 creator A5045895068 @default.
- W3024778851 creator A5079160904 @default.
- W3024778851 date "2018-04-08" @default.
- W3024778851 modified "2023-09-23" @default.
- W3024778851 title "[Analysis of 2, 3-pyridinedicarboximide and its products conversed by engineering strain with high performance liquid chromatography]." @default.
- W3024778851 doi "https://doi.org/10.3724/sp.j.1123.2017.09039" @default.
- W3024778851 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30136527" @default.
- W3024778851 hasPublicationYear "2018" @default.
- W3024778851 type Work @default.
- W3024778851 sameAs 3024778851 @default.
- W3024778851 citedByCount "0" @default.
- W3024778851 crossrefType "journal-article" @default.
- W3024778851 hasAuthorship W3024778851A5045895068 @default.
- W3024778851 hasAuthorship W3024778851A5079160904 @default.
- W3024778851 hasConcept C119128265 @default.
- W3024778851 hasConcept C178790620 @default.
- W3024778851 hasConcept C179998833 @default.
- W3024778851 hasConcept C184651966 @default.
- W3024778851 hasConcept C185592680 @default.
- W3024778851 hasConcept C2776333044 @default.
- W3024778851 hasConcept C2777463227 @default.
- W3024778851 hasConcept C2780112151 @default.
- W3024778851 hasConcept C43617362 @default.
- W3024778851 hasConcept C7623868 @default.
- W3024778851 hasConceptScore W3024778851C119128265 @default.
- W3024778851 hasConceptScore W3024778851C178790620 @default.
- W3024778851 hasConceptScore W3024778851C179998833 @default.
- W3024778851 hasConceptScore W3024778851C184651966 @default.
- W3024778851 hasConceptScore W3024778851C185592680 @default.
- W3024778851 hasConceptScore W3024778851C2776333044 @default.
- W3024778851 hasConceptScore W3024778851C2777463227 @default.
- W3024778851 hasConceptScore W3024778851C2780112151 @default.
- W3024778851 hasConceptScore W3024778851C43617362 @default.
- W3024778851 hasConceptScore W3024778851C7623868 @default.
- W3024778851 hasIssue "4" @default.
- W3024778851 hasLocation W30247788511 @default.
- W3024778851 hasOpenAccess W3024778851 @default.
- W3024778851 hasPrimaryLocation W30247788511 @default.
- W3024778851 hasRelatedWork W1913148260 @default.
- W3024778851 hasRelatedWork W2059732763 @default.
- W3024778851 hasRelatedWork W2060083339 @default.
- W3024778851 hasRelatedWork W2069983925 @default.
- W3024778851 hasRelatedWork W2084834562 @default.
- W3024778851 hasRelatedWork W2282687875 @default.
- W3024778851 hasRelatedWork W2340263105 @default.
- W3024778851 hasRelatedWork W2517642497 @default.
- W3024778851 hasRelatedWork W2950876394 @default.
- W3024778851 hasRelatedWork W3085245795 @default.
- W3024778851 hasVolume "36" @default.
- W3024778851 isParatext "false" @default.
- W3024778851 isRetracted "false" @default.
- W3024778851 magId "3024778851" @default.
- W3024778851 workType "article" @default.