Matches in SemOpenAlex for { <https://semopenalex.org/work/W3024780863> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W3024780863 abstract "The detection, in situ or in tissue extracts, of post-translationally modified isoforms of a host-encoded protein, PrP, is diagnostic for scrapie, bovine spongiform encephalopathy (BSE) and the other transmissible spongiform encephalopathies (TSEs). Specific PrP gene mutations and polymorphisms have been linked to the incidence of naturally occuring TSEs in sheep and man, and to predisposition to experimental disease in sheep and mice. Initial restriction fragment length polymorphism (RFLP) analyses of bovine genomic DNA with the enzyme PvuII failed to establish a PrP genotype linkage with the incidence of BSE. Bovine PrP genes were then amplified by the polymerase chain reaction (PCR), cloned, and sequences obtained which agreed with previously published data. Bovine alleles contain a cytosine or thymidine at base 576 (the switch is silent) and encode five or six copies of an octarepeat motif, but no linkage with these polymorphisms was observed from studies of a large dairy herd with multiple BSE cases. Direct automated sequencing from PCR amplified genes was developed and validated by comparing results with manually derived sequences. No new polymorphisms were identified following sequencing of (6) experimental and (6) natural BSE cases, and (6) normal cows. Sequencing the PrP genes of pig and kudu, species also exposed to contaminated feed but with apparent differing susceptibilities, was performed. The pig gene has five copies of the octarepeat motif and encodes a protein of 257 amino acids, with unique residues preceding the attachment of a possible glycosylphosphatidylinositol anchor. The kudu alleles contained five or six octarepeats, encoding proteins of 256 and 264 amino acids respectively, with four amino acid differences between the alleles within the octarepeat region. This novel occurrence may be significantly linked to the kudu's apparent susceptibility. Overall, comparison of the genes from different species demonstrated that primary and predicted secondary structures were highly conserved." @default.
- W3024780863 created "2020-05-21" @default.
- W3024780863 creator A5059489144 @default.
- W3024780863 date "1993-01-01" @default.
- W3024780863 modified "2023-09-28" @default.
- W3024780863 title "Studies on PrP genes from different animal species" @default.
- W3024780863 hasPublicationYear "1993" @default.
- W3024780863 type Work @default.
- W3024780863 sameAs 3024780863 @default.
- W3024780863 citedByCount "0" @default.
- W3024780863 crossrefType "dissertation" @default.
- W3024780863 hasAuthorship W3024780863A5059489144 @default.
- W3024780863 hasConcept C104317684 @default.
- W3024780863 hasConcept C135763542 @default.
- W3024780863 hasConcept C142724271 @default.
- W3024780863 hasConcept C153911025 @default.
- W3024780863 hasConcept C168007829 @default.
- W3024780863 hasConcept C17757408 @default.
- W3024780863 hasConcept C180754005 @default.
- W3024780863 hasConcept C2776735649 @default.
- W3024780863 hasConcept C2779134260 @default.
- W3024780863 hasConcept C2781131914 @default.
- W3024780863 hasConcept C3019804061 @default.
- W3024780863 hasConcept C49105822 @default.
- W3024780863 hasConcept C53345823 @default.
- W3024780863 hasConcept C54355233 @default.
- W3024780863 hasConcept C552990157 @default.
- W3024780863 hasConcept C71924100 @default.
- W3024780863 hasConcept C86803240 @default.
- W3024780863 hasConceptScore W3024780863C104317684 @default.
- W3024780863 hasConceptScore W3024780863C135763542 @default.
- W3024780863 hasConceptScore W3024780863C142724271 @default.
- W3024780863 hasConceptScore W3024780863C153911025 @default.
- W3024780863 hasConceptScore W3024780863C168007829 @default.
- W3024780863 hasConceptScore W3024780863C17757408 @default.
- W3024780863 hasConceptScore W3024780863C180754005 @default.
- W3024780863 hasConceptScore W3024780863C2776735649 @default.
- W3024780863 hasConceptScore W3024780863C2779134260 @default.
- W3024780863 hasConceptScore W3024780863C2781131914 @default.
- W3024780863 hasConceptScore W3024780863C3019804061 @default.
- W3024780863 hasConceptScore W3024780863C49105822 @default.
- W3024780863 hasConceptScore W3024780863C53345823 @default.
- W3024780863 hasConceptScore W3024780863C54355233 @default.
- W3024780863 hasConceptScore W3024780863C552990157 @default.
- W3024780863 hasConceptScore W3024780863C71924100 @default.
- W3024780863 hasConceptScore W3024780863C86803240 @default.
- W3024780863 hasLocation W30247808631 @default.
- W3024780863 hasOpenAccess W3024780863 @default.
- W3024780863 hasPrimaryLocation W30247808631 @default.
- W3024780863 hasRelatedWork W1482119189 @default.
- W3024780863 hasRelatedWork W1965760255 @default.
- W3024780863 hasRelatedWork W1970821776 @default.
- W3024780863 hasRelatedWork W1977393442 @default.
- W3024780863 hasRelatedWork W1988392265 @default.
- W3024780863 hasRelatedWork W1988447961 @default.
- W3024780863 hasRelatedWork W1993926127 @default.
- W3024780863 hasRelatedWork W1997103907 @default.
- W3024780863 hasRelatedWork W2009927673 @default.
- W3024780863 hasRelatedWork W2018202406 @default.
- W3024780863 hasRelatedWork W2022306745 @default.
- W3024780863 hasRelatedWork W2034033231 @default.
- W3024780863 hasRelatedWork W2057955407 @default.
- W3024780863 hasRelatedWork W2090698362 @default.
- W3024780863 hasRelatedWork W2093597012 @default.
- W3024780863 hasRelatedWork W2123585193 @default.
- W3024780863 hasRelatedWork W2137613405 @default.
- W3024780863 hasRelatedWork W2418122966 @default.
- W3024780863 hasRelatedWork W2037551360 @default.
- W3024780863 hasRelatedWork W2406213543 @default.
- W3024780863 isParatext "false" @default.
- W3024780863 isRetracted "false" @default.
- W3024780863 magId "3024780863" @default.
- W3024780863 workType "dissertation" @default.