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• W3024788504 abstract "Based on the transcriptome data, the study cloned full-length cDNA of TwGPPS1 and TwGPPS2 genes from Tripterygium wilfordii suspension cells and then analyzed the bioinformation of the sequence and protein expression. The cloned TwGPPS1 has a 1 278 bp open reading frame (ORF) encoding a polypeptide of 425 amino acids. The deduced isoelectric point (pI) was 6.68, a calculated molecular weight was about 47.189 kDa. The full-length cDNA of the TwGPPS2 contains a 1 269 bp open reading frame (ORF) encoding a polypeptide of 422 amino acids. The deduced isoelectric point (pI) was 6.71, a calculated molecular weight was about 46.774 kDa.The entire reading frame of TwGPPS1,2 was cloned into the pET-32a(+) vector and expressed in E. coli BL21 (DE3) cells to obtain the TwGPPS protein, which laid a basis for further study on the regulation of terpenoid secondary metabolism and biological synthesis." @default.
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• W3024788504 date "2017-01-01" @default.
• W3024788504 modified "2023-09-23" @default.
• W3024788504 title "[Cloning and protein expression analysis of geranyl diphosphate synthase genes in Tripterygium wilfordii]." @default.
• W3024788504 doi "https://doi.org/10.19540/j.cnki.cjcmm.20161222.042" @default.
• W3024788504 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28948723" @default.
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