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- W3024924162 abstract "Enzymes have a wide range of applications and great industrial potential. However, large-scale applications of enzymes are restricted by the harsh industrial environment, such as high temperature, strong acid/alkali, high salt, organic solvents, and high substrate concentration. Adaptive modification (such as rational or semi-rational design, directed evolution and immobilization) is the most common strategy to improve the catalysis of enzymes under industrial conditions. Here, we review the catalysis of enzymes in the industrial environment and various methods adopted for the adaptive modifications in recent years, to provide reference for the adaptive modifications of enzymes.酶在工业上有着广泛应用和巨大潜力，但工业生产中高温、强酸/碱、高盐、有机溶剂和高底物浓度等条件仍然制约着酶的大规模应用。为使酶能更好地在工业环境下发挥催化作用，目前的主要策略是对酶进行适应性改造 (如理性或半理性设计、定向进化、固定化等)。文中简要阐述了酶在工业环境下的催化行为以及近年对其适应性改造的研究进展，以期为酶的适应性改造提供参考。." @default.
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- W3024924162 date "2019-10-25" @default.
- W3024924162 modified "2023-09-23" @default.
- W3024924162 title "[Catalysis of enzymes under industrial environment and their adaptive modifications: a review]." @default.
- W3024924162 doi "https://doi.org/10.13345/j.cjb.190258" @default.
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