Matches in SemOpenAlex for { <https://semopenalex.org/work/W3024927332> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W3024927332 abstract "Abstract SARS-CoV-2, a novel coronavirus infecting humans, is responsible for the current COVID-19 global pandemic. If several strains could be isolated worldwide, especially for in-vitro drug susceptibility testing and vaccine development, few laboratories routinely isolate SARS-CoV-2. This is due to the fact that the current co-culture strategy is highly time consuming and requires working in a biosafety level 3 laboratory. In this work, we present a new strategy based on high content screening automated microscopy (HCS) allowing large scale isolation of SARS-CoV-2 from clinical samples in 1 week. A randomized panel of 104 samples, including 72 tested positive by RT-PCR and 32 tested negative, were processed with our HCS procedure and were compared to the classical isolation procedure. Isolation rate was 43 % with both strategies on RT-PCR positive samples, and was correlated with the initial RNA viral load in the samples, where we obtained a positivity threshold of 27 Ct. Co-culture delays were shorter with HCS strategy, where 80 % of the positive samples were recovered by the third day of co-culture, as compared to only 25 % with the classic strategy. Moreover, only the HCS strategy allowed us to recover all the positive elements after 1 week of co-culture. This system allows rapid and automated screening of clinical samples with minimal operator work load, thus reducing the risks of contamination." @default.
- W3024927332 created "2020-05-21" @default.
- W3024927332 creator A5012991166 @default.
- W3024927332 creator A5020915591 @default.
- W3024927332 creator A5023619971 @default.
- W3024927332 creator A5047690752 @default.
- W3024927332 creator A5070068983 @default.
- W3024927332 creator A5074730004 @default.
- W3024927332 creator A5086794879 @default.
- W3024927332 date "2020-05-19" @default.
- W3024927332 modified "2023-09-26" @default.
- W3024927332 title "High speed large scale automated isolation of SARS-CoV-2 from clinical samples using miniaturized co-culture coupled with high content screening" @default.
- W3024927332 cites W2949508971 @default.
- W3024927332 cites W3001897055 @default.
- W3024927332 cites W3003637715 @default.
- W3024927332 cites W3005212621 @default.
- W3024927332 cites W3006645647 @default.
- W3024927332 cites W3008763357 @default.
- W3024927332 cites W3009170734 @default.
- W3024927332 cites W3009219040 @default.
- W3024927332 cites W3010673910 @default.
- W3024927332 cites W3010930696 @default.
- W3024927332 cites W3011032288 @default.
- W3024927332 cites W3013013063 @default.
- W3024927332 cites W3014249633 @default.
- W3024927332 cites W3014281460 @default.
- W3024927332 cites W3014853902 @default.
- W3024927332 cites W3017617595 @default.
- W3024927332 cites W3098082307 @default.
- W3024927332 cites W4205260312 @default.
- W3024927332 doi "https://doi.org/10.1101/2020.05.14.097295" @default.
- W3024927332 hasPublicationYear "2020" @default.
- W3024927332 type Work @default.
- W3024927332 sameAs 3024927332 @default.
- W3024927332 citedByCount "3" @default.
- W3024927332 countsByYear W30249273322020 @default.
- W3024927332 countsByYear W30249273322021 @default.
- W3024927332 crossrefType "posted-content" @default.
- W3024927332 hasAuthorship W3024927332A5012991166 @default.
- W3024927332 hasAuthorship W3024927332A5020915591 @default.
- W3024927332 hasAuthorship W3024927332A5023619971 @default.
- W3024927332 hasAuthorship W3024927332A5047690752 @default.
- W3024927332 hasAuthorship W3024927332A5070068983 @default.
- W3024927332 hasAuthorship W3024927332A5074730004 @default.
- W3024927332 hasAuthorship W3024927332A5086794879 @default.
- W3024927332 hasBestOaLocation W30249273321 @default.
- W3024927332 hasConcept C126322002 @default.
- W3024927332 hasConcept C142462285 @default.
- W3024927332 hasConcept C142724271 @default.
- W3024927332 hasConcept C159047783 @default.
- W3024927332 hasConcept C2522874641 @default.
- W3024927332 hasConcept C2775941552 @default.
- W3024927332 hasConcept C2779134260 @default.
- W3024927332 hasConcept C3007834351 @default.
- W3024927332 hasConcept C3008058167 @default.
- W3024927332 hasConcept C3020328616 @default.
- W3024927332 hasConcept C52384281 @default.
- W3024927332 hasConcept C524204448 @default.
- W3024927332 hasConcept C70721500 @default.
- W3024927332 hasConcept C71924100 @default.
- W3024927332 hasConcept C86803240 @default.
- W3024927332 hasConcept C89423630 @default.
- W3024927332 hasConceptScore W3024927332C126322002 @default.
- W3024927332 hasConceptScore W3024927332C142462285 @default.
- W3024927332 hasConceptScore W3024927332C142724271 @default.
- W3024927332 hasConceptScore W3024927332C159047783 @default.
- W3024927332 hasConceptScore W3024927332C2522874641 @default.
- W3024927332 hasConceptScore W3024927332C2775941552 @default.
- W3024927332 hasConceptScore W3024927332C2779134260 @default.
- W3024927332 hasConceptScore W3024927332C3007834351 @default.
- W3024927332 hasConceptScore W3024927332C3008058167 @default.
- W3024927332 hasConceptScore W3024927332C3020328616 @default.
- W3024927332 hasConceptScore W3024927332C52384281 @default.
- W3024927332 hasConceptScore W3024927332C524204448 @default.
- W3024927332 hasConceptScore W3024927332C70721500 @default.
- W3024927332 hasConceptScore W3024927332C71924100 @default.
- W3024927332 hasConceptScore W3024927332C86803240 @default.
- W3024927332 hasConceptScore W3024927332C89423630 @default.
- W3024927332 hasLocation W30249273321 @default.
- W3024927332 hasLocation W30249273322 @default.
- W3024927332 hasLocation W30249273323 @default.
- W3024927332 hasOpenAccess W3024927332 @default.
- W3024927332 hasPrimaryLocation W30249273321 @default.
- W3024927332 hasRelatedWork W3084704945 @default.
- W3024927332 hasRelatedWork W3112467669 @default.
- W3024927332 hasRelatedWork W3127693599 @default.
- W3024927332 hasRelatedWork W3135714043 @default.
- W3024927332 hasRelatedWork W3153825676 @default.
- W3024927332 hasRelatedWork W3186502799 @default.
- W3024927332 hasRelatedWork W34080316 @default.
- W3024927332 hasRelatedWork W4210401150 @default.
- W3024927332 hasRelatedWork W4291397000 @default.
- W3024927332 hasRelatedWork W4385264226 @default.
- W3024927332 isParatext "false" @default.
- W3024927332 isRetracted "false" @default.
- W3024927332 magId "3024927332" @default.
- W3024927332 workType "article" @default.