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- W3024963882 abstract "The aim of this article is to study the regulatory feedback loop between β-catenin and IQ motif containing GTPase activating protein 1 (IQGAP1), as well as the effect of this regulation loop in colon cancer cell proliferation. Western blot was used to detect the expression of IQGAP1 and β-catenin after changing their expression respectively by transfection in SW1116 cells. CCK-8 cell proliferation assay was used to detect the effect of IQGAP1 involved in the proliferation of SW1116 cells promoted by β-catenin. The results of Western blot indicated that β-catenin could positively regulate IQGAP1, while IQGAP1 silencing could up-regulate β-catenin, forming a negative feedback loop. The results of CCK-8 showed that IQGAP1 silencing inhibited β-catenin-mediated proliferation in SW1116 cells. In conclusion, our research reveals a negative regulatory feedback loop between β-catenin and IQGAP1 which has a remarkable effect on the proliferation ability of colon cancer cells.本文主要研究 β 连环蛋白（β-catenin）和含 IQ 基序的 GTP 激酶活化蛋白 1（IQGAP1）的相互调控，及其在肠癌细胞增殖中的功能。利用细胞转染技术改变肠癌细胞 SW1116 中的 β-catenin 或者 IQGAP1 表达后，通过蛋白质印迹法分别检测 SW1116 细胞中 IQGAP1 和 β-catenin 的表达情况。利用 CCK-8 细胞增殖实验检测干扰下调 IQGAP1 对 β-catenin 促进 SW1116 细胞增殖的影响。结果发现在 SW1116 细胞中干扰 β-catenin 可以下调 IQGAP1 表达，过表达 β-catenin 可以上调 IQGAP1 并加强有丝分裂原激活蛋白激酶（MAPK）通路；干扰 IQGAP1 在 SW1116 细胞中反而引起 β-catenin 上升；同时 CCK-8 细胞增殖实验发现干扰 IQGAP1 减弱了 β-catenin 的促肠癌细胞增殖作用。本研究表明 β-catenin 与 IQGAP1 形成负向反馈调控环路，在调控肠癌细胞的增殖中发挥着重要功能。." @default.
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- W3024963882 date "2018-02-25" @default.
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- W3024963882 title "[A β-catenin/IQGAP1 regulatory feedback loop and its effects on the proliferation of colon cancer cells]." @default.
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- W3024963882 doi "https://doi.org/10.7507/1001-5515.201701041" @default.
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