FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3025080949> ?p ?o ?g. }

• W3025080949 endingPage "1070" @default.
• W3025080949 startingPage "1065" @default.
• W3025080949 abstract "To isolate tumor stem-like cells from human epithelial ovarian cancer SKOV3 cells and explore their role in the formation of vascularization mimicry (VM).SKOV3 cells were passaged to the 7th generation by suspension culture in serum-free medium, and the percentages of CD133- and CD117-positive cells in the 1st, 3rd, 5th and 7th generations were analyzed using flow cytometry. The proliferative activity of the cells sorted from the 7th generation SKOV3 cells was assessed with colony formation assay. A three-dimensional cell culture model was established to compare the ability of VM formation between the sorted cells and the parental SKOV3 cells. The expression levels of matrix metalloproteinases-2 (MMP-2) and MMP-9 in the two groups were detected using real-time PCR and Western blotting.Some SKOV3 cells formed typical cell spheres with suspension growth in serum-free medium and were passaged to the 7th generation. Flow cytometry revealed that the percentage of CD133-positive cells increased with cell passaging. The cloning efficiency of the sorted cells was significantly higher than that of the parental SKOV3 cells (50.33% vs 5.33%, P < 0.001). The VM formation ability of the sorted cells was stronger than that of the parental SKOV3 cells in the three-dimensional cell culture system. RT-PCR and Western blotting showed that the expression levels of MMP-2 and MMP-9 were significantly higher in the 7th passage cells than in the parental cells (P < 0.05).The sorted cells from SKOV3 cells cultured in serum-free medium exhibit biological properties of tumor stem cells with strong VM formation ability, suggesting their role in VM formation." @default.
• W3025080949 created "2020-05-21" @default.
• W3025080949 creator A5001916461 @default.
• W3025080949 creator A5025001259 @default.
• W3025080949 creator A5034896367 @default.
• W3025080949 creator A5046376914 @default.
• W3025080949 creator A5086306090 @default.
• W3025080949 date "2019-09-30" @default.
• W3025080949 modified "2023-10-18" @default.
• W3025080949 title "[Role of ovarian tumor stem-like cells sorted from human epithelial ovarian cancer SKOV3 cells in vasculogenic mimicry formation]." @default.
• W3025080949 cites W1192869794 @default.
• W3025080949 cites W1525804167 @default.
• W3025080949 cites W1629342820 @default.
• W3025080949 cites W1979114728 @default.
• W3025080949 cites W1984205889 @default.
• W3025080949 cites W1988423726 @default.
• W3025080949 cites W1989769057 @default.
• W3025080949 cites W1998748259 @default.
• W3025080949 cites W2006151732 @default.
• W3025080949 cites W2013523306 @default.
• W3025080949 cites W2014862104 @default.
• W3025080949 cites W2028593825 @default.
• W3025080949 cites W2042386784 @default.
• W3025080949 cites W2047209584 @default.
• W3025080949 cites W2048988063 @default.
• W3025080949 cites W2054002500 @default.
• W3025080949 cites W2076046704 @default.
• W3025080949 cites W2106864704 @default.
• W3025080949 cites W2118019849 @default.
• W3025080949 cites W2126496132 @default.
• W3025080949 cites W2137804621 @default.
• W3025080949 cites W2143017330 @default.
• W3025080949 cites W2144514451 @default.
• W3025080949 cites W2157000170 @default.
• W3025080949 cites W2161004756 @default.
• W3025080949 cites W2161708429 @default.
• W3025080949 cites W2163798887 @default.
• W3025080949 cites W2268909667 @default.
• W3025080949 cites W2280957887 @default.
• W3025080949 cites W2316017990 @default.
• W3025080949 cites W2320582268 @default.
• W3025080949 cites W2413245610 @default.
• W3025080949 cites W2507889966 @default.
• W3025080949 cites W251101325 @default.
• W3025080949 cites W2594906601 @default.
• W3025080949 cites W2605994569 @default.
• W3025080949 cites W2606270057 @default.
• W3025080949 cites W2606887987 @default.
• W3025080949 cites W2755084001 @default.
• W3025080949 cites W2764029261 @default.
• W3025080949 cites W2773775091 @default.
• W3025080949 cites W2775823627 @default.
• W3025080949 cites W2783792790 @default.
• W3025080949 cites W2784240464 @default.
• W3025080949 cites W2785608148 @default.
• W3025080949 cites W2789922954 @default.
• W3025080949 cites W2791333129 @default.
• W3025080949 cites W2793094097 @default.
• W3025080949 cites W2805664667 @default.
• W3025080949 cites W2955341656 @default.
• W3025080949 cites W2979585789 @default.
• W3025080949 cites W75432311 @default.
• W3025080949 doi "https://doi.org/10.12122/j.issn.1673-4254.2019.09.10" @default.
• W3025080949 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6881730" @default.
• W3025080949 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31640948" @default.
• W3025080949 hasPublicationYear "2019" @default.
• W3025080949 type Work @default.
• W3025080949 sameAs 3025080949 @default.
• W3025080949 citedByCount "4" @default.
• W3025080949 countsByYear W30250809492020 @default.
• W3025080949 countsByYear W30250809492021 @default.
• W3025080949 countsByYear W30250809492022 @default.
• W3025080949 crossrefType "journal-article" @default.
• W3025080949 hasAuthorship W3025080949A5001916461 @default.
• W3025080949 hasAuthorship W3025080949A5025001259 @default.
• W3025080949 hasAuthorship W3025080949A5034896367 @default.
• W3025080949 hasAuthorship W3025080949A5046376914 @default.
• W3025080949 hasAuthorship W3025080949A5086306090 @default.
• W3025080949 hasConcept C104317684 @default.
• W3025080949 hasConcept C121608353 @default.
• W3025080949 hasConcept C1491633281 @default.
• W3025080949 hasConcept C153911025 @default.
• W3025080949 hasConcept C16685009 @default.
• W3025080949 hasConcept C185592680 @default.
• W3025080949 hasConcept C2779013556 @default.
• W3025080949 hasConcept C2779944901 @default.
• W3025080949 hasConcept C28328180 @default.
• W3025080949 hasConcept C54355233 @default.
• W3025080949 hasConcept C553184892 @default.
• W3025080949 hasConcept C55493867 @default.
• W3025080949 hasConcept C71924100 @default.
• W3025080949 hasConcept C81885089 @default.
• W3025080949 hasConcept C85265743 @default.
• W3025080949 hasConcept C86803240 @default.
• W3025080949 hasConcept C95444343 @default.
• W3025080949 hasConceptScore W3025080949C104317684 @default.
• W3025080949 hasConceptScore W3025080949C121608353 @default.
• W3025080949 hasConceptScore W3025080949C1491633281 @default.

• next