FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3025101492> ?p ?o ?g. }

• W3025101492 endingPage "742" @default.
• W3025101492 startingPage "736" @default.
• W3025101492 abstract "To investigate the influence of endoplasmic reticulum stress (ERS) on smoking-induced nucleus pulposus cells apoptosis and inflammatory response.Between October 2016 and October 2018, 25 patients with cervical disc herniation receiving discectomy were collected and divided into smoking group (14 cases) and non-smoking group (11 cases). The baseline data of age, gender, herniated segment, and Pfirrmann grading showed no significant difference between the two groups ( P>0.05). The obtained nucelus pulposus tissues were harvested to observe the cell apoptosis via detecting the apoptosis-related proteins (Caspase-3 and PRAP) by TUNEL staining and Western blot test. The nucleus pulposus cells were isolated and cultured with enzyme digestion, of which the third generation cells were used in follow-up experiments. Then, the expressions of inflammatory factors [interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α)] were detected by ELISA; the nuclear translocation of P65 was monitored by cell immunofluorescence staining. Furthermore, ERS-related proteins (GRP78 and CHOP) were detected by Western blot; and endoplasmic reticulum ultrastructure was observed under transmission electron microscope. To verify the regulatory effect of ERS, cells were pretreated by ERS specific inhibitor (4-PBA), then cell apoptosis and inflammatory response were tested.The nucleus pulposus tissue observation showed that the cell apoptotic rate and the expressions of apoptosis-related proteins (Caspase-3 and PARP) were obviously higher in smoking group than in non-smoking group ( P<0.05). The nucleus pulposus cells observation indicated that the expressions of the inflammatory factors (IL-1β and TNF-α) and the ERS-related proteins (GRP78 and CHOP) were also higher in smoking group than in non-smoking group ( P<0.05). The results of cell immunofluorescence staining further confirmed that smoking stimulated nuclear translocation of P65 in nucleus pulposus cells. The ERS injury was much more serious in smoking group than in non-smoking group. Furthermore, after 4-PBA inhibiting ERS, the expressions of GRP78, CHOP, IL-1β, TNF-α, and P65 were significantly decreased ( P<0.05), and flow cytometry results showed that cell apoptotic rate in smoking group was decreased, showing significant difference compared with the non-smoking group ( P<0.05).Somking can stimulate cell apoptosis and inflammatory response in nucleus pulposus cells via ESR pathway. Suppressing ESR may be a novel target to suspend smoking-induced intervertebral disc degeneration.探讨内质网应激对吸烟诱导的髓核细胞凋亡与炎性反应的影响。.以 2016 年 10 月—2018 年 10 月 25 例接受椎间盘摘除术的颈椎间盘突出症患者为研究对象，分为吸烟组（14 例）和非吸烟组（11 例）。两组患者年龄、性别、突出节段、Pfirrmann 分级比较，差异均无统计学意义（ P>0.05）。将术中获取的髓核组织，行 TUNEL 染色和 Western blot 检测，观察细胞凋亡情况。然后，采用酶序贯消化法分离培养髓核细胞并传代，取第 3 代细胞行 ELISA 检测炎性因子 IL-1β 和 TNF-α 含量，免疫荧光染色观察细胞中 P65 核转移情况，Western blot 检测内质网应激相关蛋白 GRP78 和 CHOP 表达，透射电镜观察细胞内质网超微结构。最后，为验证内质网调控作用，采用特异性抑制剂 4-PBA 处理吸烟组髓核细胞后，Western blot 检测 GRP78、CHOP、IL-1β、TNF-α 及 P65 蛋白相对表达量，流式细胞术检测细胞凋亡率；并以吸烟组未作处理的髓核细胞作对照。.髓核组织观测显示，吸烟组细胞凋亡率及凋亡相关蛋白 Caspase-3 和 PARP 相对表达量均明显高于非吸烟组（ P<0.05）。髓核细胞观测显示，吸烟组髓核细胞分泌 IL-1β、TNF-α 水平以及 GRP78、CHOP 蛋白相对表达量均明显高于非吸烟组（ P<0.05）；免疫荧光染色示吸烟组细胞 P65 主要表达在细胞核，而非吸烟组在细胞质；透射电镜观察显示，与非吸烟组相比，吸烟组内质网处于应激损伤状态。吸烟组髓核细胞经 4-PBA 处理后，GRP78、CHOP、IL-1β、TNF-α 及 P65 蛋白相对表达量以及细胞凋亡率均较处理前明显降低（ P<0.05）。.吸烟可通过内质网应激导致髓核细胞凋亡和炎性反应加剧，抑制内质网应激有望成为延缓吸烟导致椎间盘退变的新靶点。." @default.
• W3025101492 created "2020-05-21" @default.
• W3025101492 creator A5000772598 @default.
• W3025101492 creator A5018802446 @default.
• W3025101492 creator A5059077365 @default.
• W3025101492 creator A5064801815 @default.
• W3025101492 creator A5085279871 @default.
• W3025101492 date "2019-06-15" @default.
• W3025101492 modified "2023-09-24" @default.
• W3025101492 title "[Influence of endoplasmic reticulum stress on smoking-induced nucleus pulposus cells apoptosis and inflammatory response]." @default.
• W3025101492 cites W2189695522 @default.
• W3025101492 cites W2214858649 @default.
• W3025101492 cites W2558513068 @default.
• W3025101492 cites W2561272575 @default.
• W3025101492 cites W2572071559 @default.
• W3025101492 cites W2581455006 @default.
• W3025101492 cites W2788914217 @default.
• W3025101492 cites W2796938368 @default.
• W3025101492 cites W2885161515 @default.
• W3025101492 cites W2889964564 @default.
• W3025101492 cites W2894619545 @default.
• W3025101492 cites W2903260100 @default.
• W3025101492 doi "https://doi.org/10.7507/1002-1892.201901094" @default.
• W3025101492 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8355760" @default.
• W3025101492 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31198003" @default.
• W3025101492 hasPublicationYear "2019" @default.
• W3025101492 type Work @default.
• W3025101492 sameAs 3025101492 @default.
• W3025101492 citedByCount "0" @default.
• W3025101492 crossrefType "journal-article" @default.
• W3025101492 hasAuthorship W3025101492A5000772598 @default.
• W3025101492 hasAuthorship W3025101492A5018802446 @default.
• W3025101492 hasAuthorship W3025101492A5059077365 @default.
• W3025101492 hasAuthorship W3025101492A5064801815 @default.
• W3025101492 hasAuthorship W3025101492A5085279871 @default.
• W3025101492 hasConcept C104317684 @default.
• W3025101492 hasConcept C142724271 @default.
• W3025101492 hasConcept C153911025 @default.
• W3025101492 hasConcept C158617107 @default.
• W3025101492 hasConcept C16685009 @default.
• W3025101492 hasConcept C17991360 @default.
• W3025101492 hasConcept C185592680 @default.
• W3025101492 hasConcept C190283241 @default.
• W3025101492 hasConcept C196795494 @default.
• W3025101492 hasConcept C203014093 @default.
• W3025101492 hasConcept C2776415932 @default.
• W3025101492 hasConcept C2780723820 @default.
• W3025101492 hasConcept C55493867 @default.
• W3025101492 hasConcept C71924100 @default.
• W3025101492 hasConcept C86803240 @default.
• W3025101492 hasConcept C95444343 @default.
• W3025101492 hasConceptScore W3025101492C104317684 @default.
• W3025101492 hasConceptScore W3025101492C142724271 @default.
• W3025101492 hasConceptScore W3025101492C153911025 @default.
• W3025101492 hasConceptScore W3025101492C158617107 @default.
• W3025101492 hasConceptScore W3025101492C16685009 @default.
• W3025101492 hasConceptScore W3025101492C17991360 @default.
• W3025101492 hasConceptScore W3025101492C185592680 @default.
• W3025101492 hasConceptScore W3025101492C190283241 @default.
• W3025101492 hasConceptScore W3025101492C196795494 @default.
• W3025101492 hasConceptScore W3025101492C203014093 @default.
• W3025101492 hasConceptScore W3025101492C2776415932 @default.
• W3025101492 hasConceptScore W3025101492C2780723820 @default.
• W3025101492 hasConceptScore W3025101492C55493867 @default.
• W3025101492 hasConceptScore W3025101492C71924100 @default.
• W3025101492 hasConceptScore W3025101492C86803240 @default.
• W3025101492 hasConceptScore W3025101492C95444343 @default.
• W3025101492 hasIssue "6" @default.
• W3025101492 hasLocation W30251014921 @default.
• W3025101492 hasOpenAccess W3025101492 @default.
• W3025101492 hasPrimaryLocation W30251014921 @default.
• W3025101492 hasRelatedWork W2347767257 @default.
• W3025101492 hasRelatedWork W2353347445 @default.
• W3025101492 hasRelatedWork W2367569507 @default.
• W3025101492 hasRelatedWork W2368125811 @default.
• W3025101492 hasRelatedWork W2384775604 @default.
• W3025101492 hasRelatedWork W2410257287 @default.
• W3025101492 hasRelatedWork W2412241396 @default.
• W3025101492 hasRelatedWork W2416823479 @default.
• W3025101492 hasRelatedWork W2758030819 @default.
• W3025101492 hasRelatedWork W2782930997 @default.
• W3025101492 hasRelatedWork W2904795850 @default.
• W3025101492 hasRelatedWork W2967199779 @default.
• W3025101492 hasRelatedWork W3024679532 @default.
• W3025101492 hasRelatedWork W3029062447 @default.
• W3025101492 hasRelatedWork W3029883649 @default.
• W3025101492 hasRelatedWork W3029915423 @default.
• W3025101492 hasRelatedWork W3030125613 @default.
• W3025101492 hasRelatedWork W3030390840 @default.
• W3025101492 hasRelatedWork W3030979518 @default.
• W3025101492 hasRelatedWork W3031532692 @default.
• W3025101492 hasVolume "33" @default.
• W3025101492 isParatext "false" @default.
• W3025101492 isRetracted "false" @default.
• W3025101492 magId "3025101492" @default.
• W3025101492 workType "article" @default.