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- W3025311903 abstract "Objective: To explore the effect of neurokinin-1 receptor small interfering RNA (NK-1R-siRNA) on the expression of inflammation factors in allergic rhinitis (AR). Methods: Twenty-four male SD rats were divided into three groups randomly (by random number table methord): NK-1R-siRNA group, negative control siRNA (NC-siRNA) group and saline group, with 8 rats in each group. SD rats were sensitized and challenged with ovalbumin (OVA) to induce AR. The rats were treated intranasally with NK-1R-siRNA, NC-siRNA or saline before and during the challenge period. The AR symptoms were observed. The levels of OVA-specific IgE were measured by enzyme-linked immunosorbent assay (ELISA). The levels of NK-1R expression in the nasal mucosal tissues were determined by real time PCR (RT-PCR) and immunohistochemistry. Antibody array was used in studying the expression of inflammation cell factors in nasal mucosa. SPSS 11.0 software was used for one-factor analysis of variance. Results: Compared with saline group, AR symptoms relived significantly in NK-1R-siRNA group (nose rubbing (31.4±8.9)/15 min vs (69.5±17.9)/15 min, sneezing (7.2±1.9)/15 min vs (23.7±9.2)/15 min, nasal secretions (7.1±2.3) mg vs (24.1±4.4) mg, t value was 38.100, 17.125, 16.837, respectively, all P<0.01), and the level of serum OVA-specific IgE was also reduced ((8.56±0.73) ng/ml vs (18.05±1.22) ng/ml, t=9.787, P<0.01). The RT-PCR and immunohistochemistry results showed that the expression of NK-1R in nasal mucosa of NK-1R-siRNA group was remarkably reduced than that of the NC-siRNA group and saline group. After the treatment of NK-1R-siRNA, the expression of interleukin (IL) 1α, IL-1β, IL-4, IL-6 and IL-13 decreased, while the interferon-γ (IFN-γ) and IL-10 increased. Conclusion: NK-1R-siRNA could regulate the release of inflammation factors in AR nasal mucosa, thus relive the allergic inflammation.目的： 探讨速激肽-1受体小干扰RNA（neurokinin-1 receptor small interfering RNA，NK-1R-siRNA）对变应性鼻炎（allergic rhinitis，AR）鼻黏膜多种炎性因子表达的影响。 方法： 将24只健康雄性SD大鼠采用随机数字表法随机分为3组，分别为NK-1R-siRNA组、阴性小干扰RNA （negative control-small interfering RNA，NC-siRNA）组和生理盐水组，每组8只。将所有大鼠用卵清蛋白（ovalbumin，OVA）建立AR动物模型，同时用NK-1R-siRNA、NC-siRNA和生理盐水滴鼻干预。观察各组大鼠的AR症状。酶联免疫吸附测定（enzyme-linked immunosorbent assay，ELISA）法检测血清中OVA特异性IgE。实时定量聚合酶链反应（real time PCR，RT-PCR）及免疫组织化学（免疫组化）法检测各组大鼠鼻黏膜中NK-1R的表达情况。通过蛋白芯片技术比较各组大鼠鼻黏膜局部炎性因子的表达。使用SPSS 11.0软件，以单因素方差分析进行数据分析。 结果： 与生理盐水组比较，NK-1R-siRNA组大鼠AR症状明显缓解[挠鼻（31.4±8.9）次/15 min比（69.5±17.9）次/15 min，喷嚏（7.2±1.9）次/15 min比（23.7±9.2）次/15 min，鼻分泌物（7.1±2.3）mg比（24.1±4.4）mg，t值分别为38.100、17.125、16.837，P值均<0.01]。与生理盐水组比较，NK-1R-siRNA组大鼠血清中OVA特异性IgE水平明显降低[（8.56±0.73）ng/ml比（18.05±1.22）ng/ml，t=9.787，P<0.01]。RT-PCR和免疫组化显示NK-1R-siRNA组大鼠鼻黏膜中NK-1R表达较NC-siRNA组和生理盐水组显著下降。蛋白芯片结果显示NK-1R-siRNA干预后，AR大鼠鼻黏膜中白细胞介素（interleukin，IL）1α、IL-1β、IL-4、IL-6、IL-13等多种炎性因子表达受到抑制，而γ干扰素（interferon-γ，IFN-γ）、IL-10的表达明显升高。 结论： NK-1R-siRNA可通过调节多种AR相关炎性因子的表达，减轻鼻黏膜局部变应性炎症。." @default.
- W3025311903 created "2020-05-21" @default.
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- W3025311903 date "2019-04-07" @default.
- W3025311903 modified "2023-09-26" @default.
- W3025311903 title "[The effect of NK-1R-siRNA on expression of inflammatory factors in allergic rhinitis]." @default.
- W3025311903 doi "https://doi.org/10.3760/cma.j.issn.1673-0860.2019.04.008" @default.
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