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- W3025347377 abstract "An organic–inorganic nanocomposite which combined mesoporous silica SBA-15 and chitosan using a carboxyl functionalized ionic liquid as the bridging agent ([email protected]) was successfully fabricated, and was used to immobilize porcine pancreas lipase (PPL) by physical adsorption, cross-linking and metal–organic coordination, respectively. The as-prepared carriers were characterized by scanning electron microscopy, Fourier transform infrared and energy-dispersive X-ray spectroscopy. Compared with immobilization onto the pure mesoporous silicon material SBA-15, all the batches of PPL immobilized onto organic–inorganic nanocomposites showed higher activity, improved stability and reusability as well as better resistance to pH and temperature changes. Among the immobilized PPLs, immobilization based on Co2+ coordination ([email protected]) produced the best enzymatic properties. The maximum immobilization efficiency and specific activity of 79.6% and 1975.8 U g−1 were obtained with [email protected], separately. More importantly, the activity of immobilized enzyme can still maintain 84.0% after 10 times of reuse. These results demonstrated that thus prepared organic–inorganic nanocomposite could be an ideal carrier for enzyme immobilization by metal–organic coordination." @default.
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- W3025347377 date "2020-08-01" @default.
- W3025347377 modified "2023-09-24" @default.
- W3025347377 title "Fabrication of an organic–inorganic nanocomposite carrier for enzyme immobilization based on metal–organic coordination" @default.
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- W3025347377 doi "https://doi.org/10.1016/j.procbio.2020.05.007" @default.
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