Matches in SemOpenAlex for { <https://semopenalex.org/work/W3025394487> ?p ?o ?g. }
- W3025394487 abstract "Calcium (Ca2+)-evoked release of neurotransmitters from synaptic vesicles requires mechanisms both to prevent un-initiated fusion of vesicles (clamping) and to trigger fusion following Ca2+-influx. The principal components involved in these processes are the vesicular fusion machinery (SNARE proteins) and the regulatory proteins, Synaptotagmin-1 and Complexin. Here, we use a reconstituted single-vesicle fusion assay under physiologically-relevant conditions to delineate a novel mechanism by which Synaptotagmin-1 and Complexin act synergistically to establish Ca2+-regulated fusion. We find that under each vesicle, Synaptotagmin-1 oligomers bind and clamp a limited number of ‘central’ SNARE complexes via the primary interface and introduce a kinetic delay in vesicle fusion mediated by the excess of free SNAREpins. This in turn enables Complexin to arrest the remaining free ‘peripheral’ SNAREpins to produce a stably clamped vesicle. Activation of the central SNAREpins associated with Synaptotagmin-1 by Ca2+ is sufficient to trigger rapid (<100 msec) and synchronous fusion of the docked vesicles." @default.
- W3025394487 created "2020-05-21" @default.
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- W3025394487 date "2020-05-13" @default.
- W3025394487 modified "2023-10-16" @default.
- W3025394487 title "Synergistic roles of Synaptotagmin-1 and complexin in calcium-regulated neuronal exocytosis" @default.
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- W3025394487 doi "https://doi.org/10.7554/elife.54506" @default.
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