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- W3025548619 endingPage "3993" @default.
- W3025548619 startingPage "3978" @default.
- W3025548619 abstract "Herein, two novel principles are proposed for the judicious design of polymersomes with ultrahigh loading efficiencies for various biomacromolecules: (1) acid-induced adsorption (AIA) for proteins and (2) affinity-enhanced attraction (AEA) for nucleic acids. The former principle (AIA) is assisted by the triazole group within the polymersome membrane. The latter principle (AEA) is based on intermolecular interactions, such as the anion−π interaction and π–π stacking, which can be achieved by the judicious design of polymersomes with structurally similar repeat units as the biomacromolecular cargo [plasmid deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)]. According to these two principles, a poly(ethylene oxide)45-b-poly((α-(cinnamoyloxymethyl)-1,2,3-triazol)caprolactone)90 (PEO45-b-PCTCL90) block copolymer was prepared and self-assembled into polymersomes by directly dispersing the polymer in water. This approach allowed simultaneous polymer self-assembly and ultrahigh biomacromolecular loading efficiencies for hemoglobin (79%), plasmid DNA (75%), and RNA (85%). The AIA and AEA principles, responsible for these high loading efficiencies, were confirmed by transmission electron microscopy and molecular dynamics computer simulations. Moreover, this novel polymersome has a biodegradable membrane with photocross-linkable groups; this solves a long-standing problem in balancing electrosteric stability and biodegradability. Finally, we demonstrated the biomedical potential of the PEO45-b-PCTCL90 polymersome. Polymersomes loaded with green fluorescent protein-encoded plasmid of different sizes were efficiently transfected into HepG2 cells without interfering the gene expression. Overall, we propose two principles for designing protein and nucleic acid vectors with ultrahigh loading capability and shed light on developing noncationic vectors with tunable biodegradability." @default.
- W3025548619 created "2020-05-21" @default.
- W3025548619 creator A5011217965 @default.
- W3025548619 creator A5049317673 @default.
- W3025548619 creator A5069699353 @default.
- W3025548619 date "2020-05-13" @default.
- W3025548619 modified "2023-10-16" @default.
- W3025548619 title "Two Principles for Polymersomes with Ultrahigh Biomacromolecular Loading Efficiencies: Acid-Induced Adsorption and Affinity-Enhanced Attraction" @default.
- W3025548619 cites W1557655479 @default.
- W3025548619 cites W1616824889 @default.
- W3025548619 cites W1928972232 @default.
- W3025548619 cites W1965251103 @default.
- W3025548619 cites W1968103744 @default.
- W3025548619 cites W1969501149 @default.
- W3025548619 cites W1969577397 @default.
- W3025548619 cites W1972398775 @default.
- W3025548619 cites W1972952884 @default.
- W3025548619 cites W1973911001 @default.
- W3025548619 cites W1974302367 @default.
- W3025548619 cites W1975449661 @default.
- W3025548619 cites W1977941050 @default.
- W3025548619 cites W1978300654 @default.
- W3025548619 cites W1979264223 @default.
- W3025548619 cites W1984418916 @default.
- W3025548619 cites W1984531996 @default.
- W3025548619 cites W1984548348 @default.
- W3025548619 cites W1990796256 @default.
- W3025548619 cites W1990919895 @default.
- W3025548619 cites W1994750981 @default.
- W3025548619 cites W2003611289 @default.
- W3025548619 cites W2003836812 @default.
- W3025548619 cites W2010839601 @default.
- W3025548619 cites W2012873027 @default.
- W3025548619 cites W2014246232 @default.
- W3025548619 cites W2018263641 @default.
- W3025548619 cites W2019613864 @default.
- W3025548619 cites W2022234371 @default.
- W3025548619 cites W2026441773 @default.
- W3025548619 cites W2027245751 @default.
- W3025548619 cites W2028322263 @default.
- W3025548619 cites W2032032896 @default.
- W3025548619 cites W2034779428 @default.
- W3025548619 cites W2037595752 @default.
- W3025548619 cites W2038379840 @default.
- W3025548619 cites W2041767251 @default.
- W3025548619 cites W2044651590 @default.
- W3025548619 cites W2049109647 @default.
- W3025548619 cites W2054613696 @default.
- W3025548619 cites W2055928148 @default.
- W3025548619 cites W2062085538 @default.
- W3025548619 cites W2066856432 @default.
- W3025548619 cites W2067151173 @default.
- W3025548619 cites W2067595260 @default.
- W3025548619 cites W2071358780 @default.
- W3025548619 cites W2072413317 @default.
- W3025548619 cites W2075717437 @default.
- W3025548619 cites W2080366302 @default.
- W3025548619 cites W2081306620 @default.
- W3025548619 cites W2088654227 @default.
- W3025548619 cites W2090616039 @default.
- W3025548619 cites W2093041840 @default.
- W3025548619 cites W2093226236 @default.
- W3025548619 cites W2102037532 @default.
- W3025548619 cites W2103734924 @default.
- W3025548619 cites W2109066617 @default.
- W3025548619 cites W2111430686 @default.
- W3025548619 cites W2115030453 @default.
- W3025548619 cites W2121501788 @default.
- W3025548619 cites W2125707023 @default.
- W3025548619 cites W2136476605 @default.
- W3025548619 cites W2137486449 @default.
- W3025548619 cites W2138915768 @default.
- W3025548619 cites W2140208773 @default.
- W3025548619 cites W2140268080 @default.
- W3025548619 cites W2153175562 @default.
- W3025548619 cites W2159277126 @default.
- W3025548619 cites W2173775586 @default.
- W3025548619 cites W2270123089 @default.
- W3025548619 cites W2297815823 @default.
- W3025548619 cites W2314944118 @default.
- W3025548619 cites W2318180916 @default.
- W3025548619 cites W2319495483 @default.
- W3025548619 cites W2319557960 @default.
- W3025548619 cites W2320482143 @default.
- W3025548619 cites W2321877594 @default.
- W3025548619 cites W2323032132 @default.
- W3025548619 cites W2324780580 @default.
- W3025548619 cites W2326418027 @default.
- W3025548619 cites W2327435330 @default.
- W3025548619 cites W2331944950 @default.
- W3025548619 cites W2332659373 @default.
- W3025548619 cites W2342810459 @default.
- W3025548619 cites W2404515555 @default.
- W3025548619 cites W2413313836 @default.
- W3025548619 cites W2467764327 @default.
- W3025548619 cites W2468626314 @default.
- W3025548619 cites W2531550383 @default.
- W3025548619 cites W2533210203 @default.