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• W3025558583 abstract "HomePlant DiseaseVol. 104, No. 11First Report of Papaya leaf curl virus Associated with Leaf Curl Disease in Cestrum nocturnum in Pakistan Previous DISEASE NOTES OPENOpen Access licenseFirst Report of Papaya leaf curl virus Associated with Leaf Curl Disease in Cestrum nocturnum in PakistanAamir Lal, Eui-Joon Kil, Kainat Rauf, Muhammad Ali, and Sukchan LeeAamir LalDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon, KoreaSearch for more papers by this author, Eui-Joon Kil†Corresponding authors: E.-J. Kil; E-mail Address: viruskil@anu.ac.kr and M. Ali; E-mail Address: ali4982@gmail.com and S. Lee; E-mail Address: cell4u@skku.eduhttp://orcid.org/0000-0002-7256-3879Department of Integrative Biotechnology, Sungkyunkwan University, Suwon, KoreaDepartment of Plant Medicals, Andong National University, Andong, KoreaSearch for more papers by this author, Kainat RaufDepartment of Life Sciences, School of Science, University of Management and Technology, Lahore, PakistanSearch for more papers by this author, Muhammad Ali†Corresponding authors: E.-J. Kil; E-mail Address: viruskil@anu.ac.kr and M. Ali; E-mail Address: ali4982@gmail.com and S. Lee; E-mail Address: cell4u@skku.eduDepartment of Life Sciences, School of Science, University of Management and Technology, Lahore, PakistanSearch for more papers by this author, and Sukchan Lee†Corresponding authors: E.-J. Kil; E-mail Address: viruskil@anu.ac.kr and M. Ali; E-mail Address: ali4982@gmail.com and S. Lee; E-mail Address: cell4u@skku.eduDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon, KoreaSearch for more papers by this authorAffiliationsAuthors and Affiliations Aamir Lal1 Eui-Joon Kil1 2 † Kainat Rauf3 Muhammad Ali3 † Sukchan Lee1 † 1Department of Integrative Biotechnology, Sungkyunkwan University, Suwon, Korea 2Department of Plant Medicals, Andong National University, Andong, Korea 3Department of Life Sciences, School of Science, University of Management and Technology, Lahore, Pakistan Published Online:31 Aug 2020https://doi.org/10.1094/PDIS-12-19-2681-PDNAboutSectionsPDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Night-blooming jasmine (Cestrum nocturnum; family Solanaceae) is one of the ornamental and medicinal plants grown in tropical and subtropical regions (Shaista and Amrita 2016). Papaya leaf curl virus (PaLCuV) (genus Begomovirus, family Geminiviridae) is a bipartite circular ssDNA molecule that causes severe disease in papaya (Byun et al. 2016) and other solanaceous crops such as tobacco and tomato (Varun et al. 2017). In June 2018, during a routine survey to record begomoviruses other than cotton-infecting viruses in Pakistan, leaf curl and leaf roll disease symptoms were observed in three jasmine plants in a house garden in Lahore (31°N, 74° E), near Sheikhupura, Punjab province, Pakistan. In addition, low infestations of whiteflies were observed on symptomatic plants. Leaf samples from all three symptomatic plants were collected. Initially, total nucleic acid was extracted from a symptomatic plant using the CTAB method (Doyle and Doyle 1990). Polymerase chain reaction (PCR) amplification using universal primers Beg-F/Beg-R (Lal et al. 2020) resulted in the amplification of ∼1 kb DNA fragment. The amplicon was cloned into the pGEM-T Easy vector (Promega, Madison, WI) and sequenced by a commercial sequencing service (Macrogen, Seoul, Korea). The BLASTn analysis revealed 98% nucleotide identity with an isolate of PaLCuV (KY978407). Based on the sequence obtained, new primers PaL11-F (5′-ACGCCATTCTCTGCTTGAGC-3′) and PaL12-R (5′-GCGTGTTTATCTGGGAGATTCA-3′) were designed to obtain the full-length DNA-A of PaLCuV. PaLCuV DNA-A of approximately 2.8 kb was successfully amplified through PCR, cloned, and sequenced (GenBank accession no. MN839534), which also showed 97% sequence identity with PaLCuV (KY978407). The remaining C. nocturnum samples were also investigated through PCR amplification and found infected with PaLCuV. Furthermore, Southern blot hybridization was carried out in which the DNA extracted from one sample was resolved in agarose gels and transferred onto nylon membranes, followed by the hybridization with a [32P]dCTP-labeled full-length clone of PaLCuV (Kil et al. 2016). The pattern of hybridization of single- and double-stranded DNA bands of different conformations was observed, thus confirming the presence of begomovirus, and sequencing results confirmed it as PaLCuV. To our knowledge, this is the first report of PaLCuV associated with leaf curl disease of C. nocturnum from Pakistan. C. nocturnum can be a reservoir for PaLCuV that could infect papaya crops in Pakistan. The Solanaceae family (to which C. nocturnum belongs) contains many economically important crops (grown in Pakistan) that could also be susceptible to the PaLCuV isolate reported in this note.The author(s) declare no conflict of interest.References:Byun, H.-S., et al. 2016. Plant Dis. 100:1958. https://doi.org/10.1094/PDIS-04-16-0424-PDN Link, ISI, Google ScholarDoyle, J. J., and Doyle, J. L. 1990. Focus 12:13. Google ScholarKil, E.-J., et al. 2016. Sci. Rep. 6:19013. https://doi.org/10.1038/srep19013 Crossref, ISI, Google ScholarLal, A., et al. 2020. Plant Dis. 104:2034. Link, ISI, Google ScholarShaista, A., and Amrita, P. 2016. J. Med. Pl. Stud. 4:13. Google ScholarVarun, P., et al. 2017. Protoplasma 254:2055. https://doi.org/10.1007/s00709-017-1126-8 Crossref, ISI, Google ScholarThe author(s) declare no conflict of interest.Funding: Funding was provided by Animal and Plant Quarantine Agency (Z-1543086-2017-21-01).DetailsFiguresLiterature CitedRelated Vol. 104, No. 11 November 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionPlants of Echinacea purpurea affected by Verticillium dahliae (A. Garibaldi et al.). Photo credit: M. L. Gullino. Spinach plant infected with Stemphylium leaf spot (K. A. Spawton et al.). Photo credit: M. T. McGrath. Metrics Article History Issue Date: 30 Oct 2020Published: 31 Aug 2020First Look: 13 May 2020Accepted: 11 May 2020 Page: 3089 Information© 2020 The American Phytopathological SocietyFundingAnimal and Plant Quarantine AgencyGrant/Award Number: Z-1543086-2017-21-01KeywordsPapaya leaf curl virusCestrum nocturnumbegomovirusgeminivirusSolanaceaeThe author(s) declare no conflict of interest.PDF downloadCited byEmergence of Asian endemic begomoviruses as a pandemic threat28 September 2022 | Frontiers in Plant Science, Vol. 13Identification of a Novel Geminivirus in Fraxinus rhynchophylla in Korea28 November 2021 | Viruses, Vol. 13, No. 12" @default.
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