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• W3025648788 abstract "To preliminarily investigate morghological changes of rabbits reshaping ear cartilage assisted by microdissection needle and explore feasibility of new therapy for ear deformity.The bilateral ears of 5 male New Zealand rabbits (aged, 5-6 months) were fixed maintaining the curvature and randomly divided into 2 groups (5 ears in each group). The ears were stimulated by microdissection needle in experimental group and were not treated with stimulation in control group. The skin reaction in the experimental group was observed immediately and at 4 weeks after stimulation. Then, the fixtures were removed at 4 weeks, and the shapes of the ears were observed. The cartilages were harvested from the ears to examined morphological changes after HE staining, and measured the chondrocyte layer thickness.All rabbits survived until the end of the experiment. The skin has healed completely after 4 weeks in experimental group. After removing fixtures, the ears in the two groups all maintained certain forms momentarily; while 24 hours later, the ears in the control group mostly recovered original form, and the ears in the experimental group still maintained certain molding form until 8 weeks. HE staining showed there were smooth cartilage and uniform distribution of cells in the control group; the matrix staining was basically consistent; and the skin was normal appearance with epidermis, dermis, and cartilage of normal aspect. But the proliferation of chondrocyte with more layers of cells were observed in the experimental group. In addition, there were degeneration and injury of cartilage cells and connective tissue with necrotic cells and inflammatory cells at needle insertion sites. The chondrocyte layer thickness was (385.714±2.027) μm in the control group and (1 594.732±1.872) μm in the experimental group, there was significant difference between the two groups ( t=-759.059, P=0.000).Rabbit ear cartilage can be effectively reshaped by microdissection needle. Proliferation of chondrocyte and changes in matrix can be found during the reshaping process.初步观察微型高频针状电极刺激兔耳软骨组织后的组织形态学变化，探讨其作为耳畸形矫治方法的可行性。.5～6 月龄雄性新西兰白兔 5 只，将双侧兔耳行塑形固定后，随机取一侧耳采用微型高频针状电极刺激耳塑形区，电极刺激条带垂直于兔耳长轴，作为实验组；另一侧不接受微型针状电极刺激，作为对照组。微型针状电极刺激后即刻及 4 周时观察实验组兔耳皮肤反应；于电极刺激后 4 周拆除固定塑形装置，观察两组兔耳塑形区形态。电极刺激 8 周后取两组兔耳软骨标本，HE 染色观察软骨细胞和基质变化，测量软骨细胞层厚度。.5 只新西兰白兔均存活至实验完成。实验组与微型电极刺激后即刻比较，4 周时皮肤已愈合。拆除固定塑形装置后即刻两组均有明显塑形效果，但 24 h 后对照组基本恢复原形态，而实验组保持一定塑形形态至 8 周。HE 染色示，对照组软骨带平滑，细胞分布均匀，表皮、真皮、软骨为正常组织学表现；实验组可见软骨细胞明显增生、增大，细胞层数增厚，微型高频电极刺激针孔处尚可见局部软骨细胞损伤变性，结缔组织中有坏死细胞及炎性细胞浸润。对照组软骨细胞层厚度为（385.714±2.027）μm，实验组为（1 594.732±1.872）μm，差异有统计学意义（ t=–759.059， P=0.000）。.微型高频针状电极刺激可有效对兔耳软骨进行重塑形，在塑形过程中软骨细胞增殖，基质也发生变化。." @default.
• W3025648788 created "2020-05-21" @default.
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• W3025648788 date "2019-05-15" @default.
• W3025648788 modified "2023-09-25" @default.
• W3025648788 title "[Preliminary study on microdissection needle-assisted ear cartilage reshaping in vivo rabbit models]." @default.
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• W3025648788 doi "https://doi.org/10.7507/1002-1892.201807032" @default.
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