Matches in SemOpenAlex for { <https://semopenalex.org/work/W3025654958> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W3025654958 endingPage "329" @default.
- W3025654958 startingPage "325" @default.
- W3025654958 abstract "Induced pluripotent stem cells (iPSC) that exhibit embryonic stem cell-like properties with unlimited self-renewal and multilineage differentiation properties, are a potential cell source in regenerative medicine and cell-based therapy. Although retroviral and lentiviral transduction methods to generate iPSC are well established, the risk of mutagenesis limits the use of these products for therapeutic applications.In this study, reprogramming of human dermal fibroblasts (NHDF) into iPSC was carried out using non-integrative Sendai virus for transduction. The iPSC clones were characterised based on the morphological changes, gene expression of pluripotency markers, and spontaneous and directed differentiation abilities into cells of different germ layers.On day 18-25 post-transduction, colonies with embryonic stem cell-like morphology were obtained. The iPSC generated were free of Sendai genome and transgene after passage 10, as confirmed by RT-PCR. NHDF-derived iPSC expressed multiple pluripotency markers in qRT-PCR and immunofluorescence staining. When cultured in suspension for 8 days, iPSC successfully formed embryoid body-like spheres. NHDF-derived iPSC also demonstrated the ability to undergo directed differentiation into ectoderm and endoderm.NHDF were successfully reprogrammed into iPSC using non-integrating Sendai virus for transduction." @default.
- W3025654958 created "2020-05-21" @default.
- W3025654958 creator A5036687729 @default.
- W3025654958 creator A5044965100 @default.
- W3025654958 creator A5072596479 @default.
- W3025654958 date "2018-12-01" @default.
- W3025654958 modified "2023-10-06" @default.
- W3025654958 title "Reprogramming human dermal fibroblast into induced pluripotent stem cells using nonintegrative Sendai virus for transduction." @default.
- W3025654958 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30580364" @default.
- W3025654958 hasPublicationYear "2018" @default.
- W3025654958 type Work @default.
- W3025654958 sameAs 3025654958 @default.
- W3025654958 citedByCount "2" @default.
- W3025654958 countsByYear W30256549582022 @default.
- W3025654958 countsByYear W30256549582023 @default.
- W3025654958 crossrefType "journal-article" @default.
- W3025654958 hasAuthorship W3025654958A5036687729 @default.
- W3025654958 hasAuthorship W3025654958A5044965100 @default.
- W3025654958 hasAuthorship W3025654958A5072596479 @default.
- W3025654958 hasConcept C104317684 @default.
- W3025654958 hasConcept C107459253 @default.
- W3025654958 hasConcept C10854531 @default.
- W3025654958 hasConcept C143948831 @default.
- W3025654958 hasConcept C145103041 @default.
- W3025654958 hasConcept C1491633281 @default.
- W3025654958 hasConcept C15152581 @default.
- W3025654958 hasConcept C153911025 @default.
- W3025654958 hasConcept C18155467 @default.
- W3025654958 hasConcept C2780127873 @default.
- W3025654958 hasConcept C28328180 @default.
- W3025654958 hasConcept C54355233 @default.
- W3025654958 hasConcept C55493867 @default.
- W3025654958 hasConcept C77255625 @default.
- W3025654958 hasConcept C86803240 @default.
- W3025654958 hasConcept C95444343 @default.
- W3025654958 hasConceptScore W3025654958C104317684 @default.
- W3025654958 hasConceptScore W3025654958C107459253 @default.
- W3025654958 hasConceptScore W3025654958C10854531 @default.
- W3025654958 hasConceptScore W3025654958C143948831 @default.
- W3025654958 hasConceptScore W3025654958C145103041 @default.
- W3025654958 hasConceptScore W3025654958C1491633281 @default.
- W3025654958 hasConceptScore W3025654958C15152581 @default.
- W3025654958 hasConceptScore W3025654958C153911025 @default.
- W3025654958 hasConceptScore W3025654958C18155467 @default.
- W3025654958 hasConceptScore W3025654958C2780127873 @default.
- W3025654958 hasConceptScore W3025654958C28328180 @default.
- W3025654958 hasConceptScore W3025654958C54355233 @default.
- W3025654958 hasConceptScore W3025654958C55493867 @default.
- W3025654958 hasConceptScore W3025654958C77255625 @default.
- W3025654958 hasConceptScore W3025654958C86803240 @default.
- W3025654958 hasConceptScore W3025654958C95444343 @default.
- W3025654958 hasIssue "3" @default.
- W3025654958 hasLocation W30256549581 @default.
- W3025654958 hasOpenAccess W3025654958 @default.
- W3025654958 hasPrimaryLocation W30256549581 @default.
- W3025654958 hasRelatedWork W1971941167 @default.
- W3025654958 hasRelatedWork W1994881679 @default.
- W3025654958 hasRelatedWork W2064342320 @default.
- W3025654958 hasRelatedWork W2075757700 @default.
- W3025654958 hasRelatedWork W2154029502 @default.
- W3025654958 hasRelatedWork W2360633515 @default.
- W3025654958 hasRelatedWork W2519697816 @default.
- W3025654958 hasRelatedWork W2560446420 @default.
- W3025654958 hasRelatedWork W2774414107 @default.
- W3025654958 hasRelatedWork W3025654958 @default.
- W3025654958 hasVolume "40" @default.
- W3025654958 isParatext "false" @default.
- W3025654958 isRetracted "false" @default.
- W3025654958 magId "3025654958" @default.
- W3025654958 workType "article" @default.