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- W3025677916 abstract "Objective: To evaluate the effect of iodinate contrast agent (ICA) on the formation of DNA double-stand breaks (DSBs) in human peripheral blood lymphocytes during computed tomography (CT) examinations. Methods: Peripheral venous blood (16 ml/person) of 36 volunteers were collected during the period of February to May 2016. Each blood sample was divided into 8 tubes and 4 groups on average, which were marked as groups of A, B, C, D randomly. 25 μl ICA was respectively added into one tube of each group and the groups of B, C, D accepted the same CT scan for once, twice and three times. The lymphocytes in these blood samples were separated by using density-gradient centrifugation, fixed and immunostained with γH2AX antibody. The average number of phosphorylated histone H2AX (γH2AX) foci per lymphocyte was counted under a fluorescence microscopy. Results: The numbers of γH2AX foci were increased approximately 82.9% ((0.064±0.025) vs (0.035±0.010) foci/cell) if ICA was simply added into the blood. With the increase of CT radiation, the foci numbers of the groups of B, C, D would increase and the samples containing ICA could have a more amount of foci numbers than those without ICA (B: (1.217±0.158) vs (0.774±0.258); C: (2.128±0.185) vs (1.569±0.201); D: (3.812±0.490) vs (2.938±0.480) foci/cell). The more radiation it had, the more significant the difference of foci numbers would be ((0.489±0.323), (0.549±0.278), (0.692±0.462) foci/cell). Conclusion: The ICA itself can lead to an increase of the DSBs level and the application of ICA can amplify the DNA damage as assessed with γH2AX foci formation during diagnostic X-ray procedures.目的： 评估CT检查中碘对比剂对人外周血单个核细胞DNA双链的影响。 方法： 采集2016年2至5月期间36名健康志愿者的外周静脉血(16 ml/人)，将其平均分成4组8管，每组其中1管加入25 μl对比剂作为对照管。4组样本随机标记为A～D组，其中B、C、D组在相同CT辐射条件下辐射1、2、3次。实验室离心获取外周血淋巴细胞，荧光显微镜下计数每个淋巴细胞中γH2AX焦点数量。 结果： A组中单纯加入碘对比剂后γH2AX焦点比基础焦点数量增加约82.9%[(0.064±0.025)和(0.035±0.010 )焦点/细胞)]；随着CT辐射剂量增加，B～D组焦点损伤数量亦增加，且同组中加入对比剂的样本比单纯辐射损伤更多[B组：(1.217±0.158)和(0.774±0.258)焦点/细胞；C组：(2.128±0.185)和(1.569±0.201)焦点/细胞；D组：(3.812±0.490)和(2.938±0.480)焦点/细胞]；在一定范围内，CT辐射剂量越大，碘对比剂放大辐射损伤效应越明显[B～D组两管焦点差值分别为(0.489±0.323)、(0.549±0.278)、(0.692±0.462)焦点/细胞]。 结论： 碘对比剂本身可能引起DNA双链断裂，在CT检查中碘对比剂的存在能够放大DNA损伤。." @default.
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- W3025677916 date "2017-06-06" @default.
- W3025677916 modified "2023-09-23" @default.
- W3025677916 title "[Research on the effect of iodinate contrast agent on the DNA radiation damage at CT]." @default.
- W3025677916 doi "https://doi.org/10.3760/cma.j.issn.0376-2491.2017.21.010" @default.
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