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- W3025705901 abstract "Objective To investigate the effect of miR-497 over-expression on the proliferation of U87 human glioma cells. Methods We packaged both pGLV3/H1-NC lentivirus as a negative control group and pGLV3/H1-miR-497 lentivirus as an experimental group, and then constructed U87-NC and U87-miR-497 cell lines, respectively. The relationship between miR-497 and neuregulin receptor degradation protein 1 (Nrdp1) was analyzed by luciferase reporter assay in U87 cells; cell colony formation assay was used to detect cell proliferation and flow cytometry to detect cell cycle; the expressions of Nrdp1, AKT and phosphorylated AKT (p-AKT) were determined by Western blotting. Results We successfully packaged pGLV3/H1-NC and pGLV3/H1-miR-497 lentivirus, and obtained stable U87-NC and U87-miR-497 cell lines. When miR-497 was over-expressed in U87 cells, the cell colony formation ability was enhanced compared with the control group U87-NC. The luciferase reporter assay confirmed that miR-497 targeted Nrdp1 in U87 cells. In the stable infected cells, the level of Nrdp1 protein decreased and p-AKT protein increased, while the AKT protein did not change significantly after over-expression of miR-497. Conclusion Over-expression of miR-497 promotes the proliferation of glioma cells U87 by targeting Nrdp1." @default.
- W3025705901 created "2020-05-21" @default.
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- W3025705901 date "2017-08-01" @default.
- W3025705901 modified "2023-09-23" @default.
- W3025705901 title "[Over-expression of miR-497 promotes the proliferation of U87 glioma cells by targeting neuregulin receptor degradation protein 1]." @default.
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